Culturing of adherent cells/tissues in a sterile natural 3-D environment continuously for over 12 months allows for impressive cell densities exceeding 10^10 all in a compact laboratory tabletop apparatus. This massive concentration of cells (up to 10,000x more than conventional systems) in the Glen Mills / Zellwerk Z®RP Reactor System opens new areas for development in medicine, classic cell and stem cell research, tissue growth for replacements, large-scale biochemical production by perfusion, and others. Equipment complies with GLP, GCP, GTP, and GMP standards.
Equipment outline - Employing turning shaft technology Z®RP overcomes failings of rotation bottles, t-flasks, fluidized beds, and other methods. The growing cells, immobilized on the ceramic support, are rotated through the air and then dipped into the medium. This motion allows for the optimal controlled perfusion of nutrients and metabolic products without shear stress.
Breeder Package - The fully integrated package consists of 500ml or 5,000ml Bioreactor® fitted with cell supports, the Breeder® which is a sterile, isothermal incubator, and Control System for long-term stable operations and data logging.
Scaffolds - you have a choice between using our scaffold material, Sponceram® or your autoligous, allogeneous or synthetic material. Adherent cells can develop nature 3-D Extracellular Matrixes (ECM) on the proven zirconium oxide based Sponceram®. Two to twenty-one discs can be installed in the Bioreactor.
Existing Applications - Several applications include the culture of umbilical cord stem cells, CHO (Chinese Hamster Ovary) production clones, chondrocytes and hepatocytes. This technology has also been used in the production of (recombinant) proteins, especially complex glycoproteins. Within the Z®RP large amounts of vital cells can be grown in a natural, 3-D state for eventual harvesting.
Cell dislodgement and disruption - It is not just a matter of growing your ECM, but we also have the techniques to continuously extract the valuable perfusion bioproducts and to release the cells from the scaffold for extraction.
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