Adsorption Equipment - UNIBOND C18, 14%C 150 pore size, 35-75m particle size Reversed Phase Silica Gel -- 78050Supplier: ichromatography/Analtech
Description: chromatography: A C18 octadecyl bonded silica gel and a hydrocarbon impregnated silica gel. The most common reversed phase adsorbent is the octadecyl (C18) bonded silica gel. The C18 hydrocarbon is chemically anchored to the silica gel support. This material is compatible with all aqueous and organic solvent
- Adsorption Media: Silica Gel
Supplier: Hamilton Co.
Description: , and is completely inert to most conditions commonly encountered in reversed-phase chromatography. The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences
- Stationary Phase/Separation Process: Reversed Phase
- Packing Material Diameter: 5 µm
- Pore Diameter: 0.0100 µm
- Operating Pressure: 0.0 to 5000 psi
Technical Journals - Detecting an Algal Toxin by High-Pressure Liquid Chromatography (PDF) -- JAW_0006740Supplier: American Water Works Association (AWWA)
Description: This article presents highlights of a study conducted to determine the applicability of high-pressure liquid chromatography (HPLC) with ultraviolet (UV) detection as a rapid screening technique for the isolation,identification, and quantification of anatoxin-a, a neurotoxin produced by certain
Standards and Technical Documents - Use of Proteomics Methodology to Evaluate Inflammatory Protein Expression in Tendinitis - BIOMED 2003
Description: with endoproteinase Glu-C and trypsin and the resulting peptide mixtures were evaluated using reverse-phase C18 HPLC and Tris-tricine SDS-polyacrylamide gel electrophoresis. The two ICAT-modified peptide populations were mixed, affinity-purified and analyzed using microcapillary liquid chromatography
Description: The basis of retention in Reversed Phase Chromatography is just the opposite of that seen in normal phase (e.g., silica gel) chromatography.In normal phase chromatography the adsorbent is a polar material. Adsorption occurs as a result of polar interactions. Thus, in normal phase chromatography
Standards and Technical Documents - API DR148 -- HEALTH AND ENVIRONMENTAL SCIENCES DEPARTMENTAL REPORT - IDENTIFICATION OF ORGANIC TOXICANTS IN TREATED REFINERY EFFLUENTSSupplier: American Petroleum Institute (API)
Description: of nonpolar organic toxicants. Nonpolar organic toxicants were operationally defined as those adsorbed by C18 SPE columns. Desirable effluent characteristics were: 1) consistent presence of measurable chronic toxicity due to nonpolar organic compounds; and 2) a lack of toxicity from compounds other
Supplier: Toronto Research Chemicals, Inc.
Description: Chromatography Media
- Applications: Pharmaceuticals / Drugs
- Form: Powder
Supplier: MAC-MOD Analytical, Inc.
Description: ProntoSIL HPLC columns from Bischoff Chromatography are available in a wide variety of bonded phases and dimensions. In addition to more typical C8 and C18 phases, ProntoSIL columns are also available in hard to find bonded phases such as C1, amino, diol and C30. If you are looking for a specialty
- Packing Material Diameter: 5 µm
- Pore Diameter: 0.0120 µm
- Column Length: 25 cm
- Internal Diameter: 20 mm
Supplier: Western Analytical Products
Description: "1.0 mm ID x 2 cm Ideal for Microbore HPLC Easily Dry Packed (or Slurry Packed with Adapter) Made of PEEK Polymer and Stainless Steel This Upchurch Scientific® ultralow volume guard column is ideal for narrow-bore chromatography. The unpacked guard column allows you to exactly match
- Column Type: Guard Column
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High-efficiency Separations At Any pH
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability. ( > 100 °C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography. The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited... (read more)
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Prior to the preparative C18 chromatography , a "fingerprint" chromatogram of each sample was taken on analytical C18 HPLC in order to determine the chlorin distribution.
Antigen Analysis of Mycobacterium tuberculosis H37Rv Culture Filtrate Proteins
Two different approaches were used to separate the culture filtrate protein mixture: (i) proteins were fractionated according to their hydrophobicity using an HPLC‐ C18 chromatography column followed by separation based on their molecular mass by SDS‐PAGE and subsequent immunoblotting or (ii) proteins …
Utilisation of chickpea protein isolates for production of peptides with angiotensin I‐converting enzyme (ACE)‐inhibitory activity
Fractions selected for further puri®cation of peptides were pooled and taken to dryness before HPLC C18 chromatography .
By purifying our nickel-purified mCD9-LEL protein over C18 chromatography , we were able to eliminate contaminating higher order oligomers, bringing our protein .
To improve the recovery yield of the product, every 10% increment of acetonitrile from 20–100% acetonitrile in water containing 0.1% TFA solvent system of the RediSep C18 chromatography was performed.
Synthesis of the Tolerance‐Inducing Oligosaccharide Lacto‐ N ‐Fucopentaose III Bearing an Activated Linker
The residue was subjected to C18 chromatography using gradient elution (0.5% aq AcOH!
High-performance metabolic profiling with dual chromatography-Fourier-transform mass spectrometry (DC-FTMS) for study of the exposome
For DC- FTMS, we combined data from sequential LC-FTMS analyses using reverse phase ( C18 ) chromatography and anion exchange (AE) chromatography.
Metabolic Biomarkers of Prenatal Alcohol Exposure in Human Embryonic Stem Cell–Derived Neural Lineages
Supernatants were subjected to C18 chromatography followed by ESI‐QTOF‐MS.
C18 chromatography column (250 mm × 25 mm i.d., 300 Å, 10 μm particle size) 20.
Colombistatin: a disintegrin isolated from the venom of the South American snake (Bothrops colombiensis ) that effectively inhibits platelet aggregation and SK...
Thirty-six fractions were collected by reverse phase C18 chromatography , and all fractions were tested for proteo- lytic activity and for inhibition of ADP-induced platelet aggregation on whole human blood.
Chromatography of serum on Sep-pak C18 corrects falsely elevated vitamin D metabolite levels measured by protein binding assay.
Sera from 5 patients (4 with osteomalacia, 1 with chronic pancreatitis/malabsorption) had vitamin D metabolite levels in the normal range after ethanol extraction but had low levels after Sep-pak C18 chromatography ; four of these sera also had low levels after chromatography …
Nuclease Methods and Protocols
(Flow rate for all Mono S and C18 chromatography described below is 0.8 mL/min.
Reduction of the nitrile groups with LAH followed by treatment with HCl yielded the deprotected free-base amine porphyrin 2.60, which was isolated through reverse phase ( C18 ) chromatography .
protein mixture analysed in paper I, using protein fractionation according to their hydrophobicity by a HPLC- C18 chromatography column and molecular mass.