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Supplier: Analtech, Inc.
Description: chromatography: A C18 octadecyl bonded silica gel and a hydrocarbon impregnated silica gel. The most common reversed phase adsorbent is the octadecyl (C18) bonded silica gel. The C18 hydrocarbon is chemically anchored to the silica gel support. This material is compatible with all aqueous and organic solvent
Supplier: Analtech, Inc.
Description: SMT offers a variety of packing materials in bulk quantities for reversed-phase, normal phase, and ion exchange chromatography. SMT's packing materials usually consists of silica substrates and are made withtotal coveragetechnology and guaranteed to last much longer than competitor's packings
Standards and Technical Documents - Use of Proteomics Methodology to Evaluate Inflammatory Protein Expression in Tendinitis - BIOMED 2003
Description: with endoproteinase Glu-C and trypsin and the resulting peptide mixtures were evaluated using reverse-phase C18 HPLC and Tris-tricine SDS-polyacrylamide gel electrophoresis. The two ICAT-modified peptide populations were mixed, affinity-purified and analyzed using microcapillary liquid chromatography
Supplier: Toronto Research Chemicals, Inc.
Description: Chromatography Media
- Applications: Pharmaceuticals / Drugs
- Form: Powder
Description: Feature an ultra-high surface area (30% carbon load) and spherically shaped C18 packing to support high-resolution chromatography of species having similar structures Adsorbosp Uhsc18,250x4.6,10u B ™Adsorbosphere™ C18 4.6 x 250mm 10µm For Equipment that meets Industry
Description: Feature an ultra-high surface area (30% carbon load) and spherically shaped C18 packing to support high-resolution chromatography of species having similar structures Adsorbosp Uhsc18, 150x4.6,5u C ™Adsorbosphere™ C18 HPLC Column 4.6 x 150mm Reversed phase 5µm Analytical
- Column Type: Analytical Column
- Stationary Phase/Separation Process: Reversed Phase
Description: Sample preparation and precise liquid handling in one device A 0.6µL bed of C18, C4, or metal chelate chromatography media is fixed at the end of a 10µL pipet tip such that there is no dead volume; concentrated, purified samples are eluted in 1 to 4µL. For applications
- Configuration: Pipette Tip
Supplier: MAC-MOD Analytical, Inc.
Description: ProntoSIL HPLC columns from Bischoff Chromatography are available in a wide variety of bonded phases and dimensions. In addition to more typical C8 and C18 phases, ProntoSIL columns are also available in hard to find bonded phases such as C1, amino, diol and C30. If you are looking for a specialty
- Packing Material Diameter: 5 µm
- Pore Diameter: 0.0100 µm
- Column Length: 2 cm
Supplier: Western Analytical Products
Description: "1.0 mm ID x 2 cm Ideal for Microbore HPLC Easily Dry Packed (or Slurry Packed with Adapter) Made of PEEK Polymer and Stainless Steel This Upchurch Scientific® ultralow volume guard column is ideal for narrow-bore chromatography. The unpacked guard column allows you to exactly match
- Column Type: Guard Column
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Featured Products for C18 Chromatography Top
High-efficiency Separations At Any pH
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability. ( > 100 °C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography. The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited... (read more)
Browse HPLC Columns Datasheets for Hamilton Co.
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USLC TM Columns Put the Right Tools in Your LC Method Development Toolbox
the selectivity needed. Table I The Fs term of the hydrophobic subtraction model (H-S model) can numerically determine which. stationary phases are most dissimilar to a C18, illustrating the phases needed to extend the selectivity range in. reversed phase chromatography. The 4 Restek USLC™ phases are shown
Faster, more cost-effective methods for generic drugs using Agilent 1290 Infinity and ISET
on an Agilent ZORBAX Eclipse Plus C18 4.6 x 150 mm, 5 um column (Part Number: 959993-902), which is the pharmacopeia-recommended column dimension. Then we selected three smaller column dimensions, which were within the EP allowed deviation limit and repeated the method on each column for experiments 2
Tips and tools for trouble-free food testing
superior results when analyzing food using columns with sub-2 um or sub-3 um particles, be sure to follow these important steps: Choose the type of chromatography - either reversed phase (C18, polar embedded, etc.) or hydrophilic interaction liquid chromatography (HILIC), which is particularly good
Rapid Analysis of Mycophenolic Acid in Human Plasma Using an Agilent Triple Quadrupole LC/MS/MS System with Automated Online Sample Cleanup
to. OH. 2. 0.20. 0.98. improve instrument robustness. MPA-G. 1. 0.10. 0.49. Figure 1. Structures for MPA and MPA-G. 2. LC parameters are listed in Tables 2, 3, 4,. Table 2. LC conditions. and 5. Parameter. Value. Automated on-line sample cleanup. Columns. Trapping: Agilent ZORBAX Eclipse Plus C18
Rapid Analysis of Cyclosporine A, Everolimus, Sirolimus, and Tacrolimus Drugs in Whole Blood Using an Agilent Triple Quadrupole LC/MS/MS System with Automated Online Sample Cleanup
a simple protein. Columns. Trapping: Agilent ZORBAX Eclipse Plus C18, 2.1 × 12.5 mm, 5 µm. precipitation procedure: (p/n 821125-936). 1. Mix 100 µL of whole blood. Analytical: Agilent Poroshell 120 EC-C18, 3 × 50 mm, 2.7 µm (p/n 699975-302). with 200 µL of precipitating. Column temperature. 60 °C
Agilent MassHunter Easy Access Software for Lot-to-Lot Purification Analysis of a Model Therapeutic Protein
, it is very important to. Column. Agilent Poroshell C18, 75 × 2.1 mm, 5 µm. analyze any change implemented in the. Column temperature. 60 °C. manufacturing process. This Application. Note describes the use of an LC coupled. Sample thermostat. 5 °C. to a high deﬁ nition Q-TOF to analyze. Mobile
More Information on: C18 Chromatography Top
Prior to the preparative C18 chromatography , a "fingerprint" chromatogram of each sample was taken on analytical C18 HPLC in order to determine the chlorin distribution.
Chromatography of serum on Sep-pak C18 corrects falsely elevated vitamin D metabolite levels measured by protein binding assay.
Sera from 5 patients (4 with osteomalacia, 1 with chronic pancreatitis/malabsorption) had vitamin D metabolite levels in the normal range after ethanol extraction but had low levels after Sep-pak C18 chromatography ; four of these sera also had low levels after chromatography...
Investigation of the Microbial Glyoxalase System
To improve the recovery yield of the product, every 10% increment of acetonitrile from 20–100% acetonitrile in water containing 0.1% TFA solvent system of the RediSep C18 chromatography was performed.
By purifying our nickel-purified mCD9-LEL protein over C18 chromatography , we were able to eliminate contaminating higher order oligomers, bringing our protein .
Nuclease Methods and Protocols
(Flow rate for all Mono S and C18 chromatography described below is 0.8 mL/min.
Colombistatin: a disintegrin isolated from the venom of the South American snake (Bothrops colombiensis ) that effectively inhibits platelet aggregation and SK...
Thirty-six fractions were collected by reverse phase C18 chromatography , and all fractions were tested for proteo- lytic activity and for inhibition of ADP-induced platelet aggregation on whole human blood.
Glycan chain length distributions are typically measured by isolating bacterial sacculi, using an amidase to break the crosslinks between the glycan strands, and then evaluating the length distribution by separating the strands using C18 chromatography .
Understanding Biology Using Peptides
The peptide was solubilized with aqueous acetic acid and purified by preparative Kromasil C18 chromatography in 0.1% TFA, with a linear acetonitrile gradient.
Bacterial Toxins: Methods and Protocols
We have found that on-line preconcentration of de-O-acylated LPS samples by adsorption onto C18 chromatography particles or SDB-XC membranes can lead to a 40-fold improvement in concentration detection limits of total de-O-acylated LPS (27).
Isolation, Characterization and Antifungal Activity of Proteinase Inhibitors from Capsicum chinense Jacq. Seeds
The obtained peptide enriched fractions (PEFs) were further separated using HPLC with a C2/C18 column (lRPC C2/C18 ST 4.6/100, GE Healthcare) and a linear propanol gradient, following the same elution con- ditions described for the above C18 chromatography .