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  • In Vitro Transcription and Translation Protocols
    Dounce homogenizer (baked).
  • RNA-Protein Interaction Protocols
    Dounce homogenizer (all glass-type) and pestles Type A and B from Wheaton (Millville, NJ, USA).
  • Mitochondrial DNA
    Transfer 40 mL of resuspended mitochondria into a Dounce homogenizer .
  • Chromatin Protocols
    Glass Dounce homogenizer .
  • mRNA Processing and Metabolism
    Homogenize on ice using a Dounce homogenizer with a B pestle to 80–100% lysis and pellet nuclei at 4°C (2000g) for 5 min. 5.
  • Organelle Proteomics
    Loose-fitting (Wheaton type B pestle) glass dounce homogenizer with clearance of between ∼0.1 and 0.15 mm. 3.
  • Chromatin Protocols
    Keep the cells on ice for 10 min in 100–200 ml of buffer A (depending on the cell pellet size, preferable to keep the buffer volume small to obtain concentrated protein) and disrupt in a type S dounce homogenizer .
  • Mitochondria
    ...CaCl2, 0.5 mM EDTA, 0.5 mM DTT, 0.28 M ultrapure sucrose, 1 mM PMSF, 10 mM sodium metabisulfite, and 2 g/mL leupeptin; homogenize in approx 7-mL portions in a standard (7-mL) Dounce homogenizer using six strokes of...
  • The Nucleus
    Muscle tissue is initially broken down by mincing, and is then gently homogenized using a motor- ized Dounce homogenizer (such as a Potter-Elvehjem).
  • Membrane Proteomics
    Dounce homogenizer with loose fitting plunger.
  • RNA-Protein Interaction Protocols
    As an alternative to the nuclear extract protocol described here, cells can be lysed on hypotonic swelling with a Dounce homog- enizer , on which the pelleted nuclei are extracted with high salt (10).
  • Connexin Methods and Protocols
    Fill the Dounce homogenizer with cold 1 mM NaHCO3 buffer (~50 mL).
  • Alzheimer’s Disease
    Homogenize the tissue with a glass Dounce homogenizer .
  • Membrane Protein Protocols
    Dounce homogenizer , tight pestle.
  • Vaccinia Virus and Poxvirology
    Resuspend the infected cell pellet in 8 mL of 10 mM Tris, and release the virus from the cells with a Dounce homogenizer .