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  • Medical Device Link . Development applications for membrane-bottom microwell plates
    , then centrifuged using a microplate rotor. Both the flow-through material and the material eluted with buffer contain pure PCR products. In the 96-well membrane-bottom plate, therefore, column chromatography of single samples evolved into spin-column chromatography of 96 samples in parallel
  • Solutions for Small Volume Instruments
    . that smaller samples can be analyzed without a loss of reliability. This is great news since many samples are. difficult to obtain, have a limited supply or are too expensive to use in large volumes. Microplate readers,. micro cuvettes and new spectrophotometer designs have allowed samples
  • Medical Device Link .
    labeling one primer with a hapten or a ligand, such as biotin, and, after amplification, capturing it with an antibody- or streptavidin-coated microplate. The other primer is labeled with a reporter such as fluorescein, and detection is achieved by adding an antifluorescein antibody, horseradish
  • Medical Device Link .
    a Turner Designs Inc. (Sunnyvale, CA) TD 20e luminometer provided with a neutral density filter to attenuate light intensity or a Thermo Labsystems (Franklin, MA) Luminoskan microplate luminometer. All chemiluminescence measurements were performed at room temperature except where specifically noted
  • Medical Device Link . Development of monoclonal antibodies and a rapid assay for tuberculosis
    antimouse IgG, A & M antibody for one hour, followed by washing. Tetramethylbenzidine substrate was pipetted into the wells and allowed to develop for 15 to 30 minutes. Development was halted by the addition of 2 M phosphoric acid and the results were read in a microplate reader at 450 nm. Immunizations