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  • Two-Photon Excited Fluorescence Microscopy
    Protein has further increased the imaging. capabilities of TPM. By fusing auto-fluorescent proteins to. Although two-photon absorption can be induced by using CW. proteins of interest, TPM can perform imaging of complex. lasers, pulsed lasers are preferable because they can achieve. cellular processes
  • Medical Device Link . Fluorescence in situ hybridization
    and immunohistochemistry (IHC) for HER-2/neu protein expression are often used to quantitate gene amplification, each presents technical and interpretative limitations. The results of IHC vary with use of different antibodies and tissue treatment, use of different criteria for positivity, and use
  • Thermal Denaturation of Hen-Egg Lysozyme with Concurrent CD and Fluorescence Detection
    temperature). Lysozyme is a common model protein used to investigate the reversible inactivation of proteins at high temperature because it is inexpensive, easy to handle, easy to denature, and has just enough complexity in the fluorescence spectra to make it interesting. Lysozyme, a small globular
  • Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
    . We also demonstrate yielded equal excitation rates in the simultaneous excitation. By the selective excitation of a donor fluorescent protein, fluorescence resonance energy transfer imaging is also achieved.
  • Medical Device Link .
    of protein microarray technologies to diagnostics. Fluorescence image of mouse DNA expression microarray. The transfer of DNA microarray methods to protein microarray diagnostics is currently under development. However, the technology transfer from DNA to protein arraying creates new challenges when
  • Medical Device Link .
    microarrays in spot size (150 mm diam) and detection methodology (e.g., fluorescence). This article examines efforts to develop multianalyte microimmunoassays, some of which have resulted in commercial products. Protein Microarray. Literature Review. The term protein microarray encompasses a large
  • Medical Device Link . Measuring microsphere binding capacity
    these magnetic microspheres preconjugated with some type of generic binding protein. A common choice is streptavidin (from , or produced recombinantly), which is similar to the avian egg protein avidin. Streptavidin interacts strongly with the molecule biotin, and so by biotinylating the ligand to be coupled
  • Medical Device Link .
    of conventional laboratories for protein diagnostics, that is, dosing, centrifugation, and purification, must be replaced by integrated polymer microcomponents. As a result, the essential component of this diagnostic platform is likely to be a disposable chip, which includes the microfluidics for blood