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  • Thermal Denaturation of Hen-Egg Lysozyme with Concurrent CD and Fluorescence Detection
    temperature). Lysozyme is a common model protein used to investigate the reversible inactivation of proteins at high temperature because it is inexpensive, easy to handle, easy to denature, and has just enough complexity in the fluorescence spectra to make it interesting. Lysozyme, a small globular
  • Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
    . We also demonstrate yielded equal excitation rates in the simultaneous excitation. By the selective excitation of a donor fluorescent protein, fluorescence resonance energy transfer imaging is also achieved.
  • Medical Device Link . Measuring microsphere binding capacity
    these magnetic microspheres preconjugated with some type of generic binding protein. A common choice is streptavidin (from , or produced recombinantly), which is similar to the avian egg protein avidin. Streptavidin interacts strongly with the molecule biotin, and so by biotinylating the ligand to be coupled
  • Medical Device Link .
    of conventional laboratories for protein diagnostics, that is, dosing, centrifugation, and purification, must be replaced by integrated polymer microcomponents. As a result, the essential component of this diagnostic platform is likely to be a disposable chip, which includes the microfluidics for blood
  • Medical Device Link .
    could help bring finger-prick blood tests to every physician's office and hospital bedside, in the process revolutionizing protein-based in vitro diagnostics. The criteria for judging these systems include cost, sample size, overall turnaround time, versatility, and portability. Specificity
  • Medical Device Link .
    , Denmark), immunoassay sensitivity and performance were observed to be higher than with standard nondextran Cy5 conjugates in which the Cy5 normally is conjugated directly to the protein. One explanation for this superiority might be reduced quenching of the dye; because the fluorophors are spread
  • Medical Device Link . Formal experimental design and analysis for immunochemical product development
    . As the example proceeds, it will look at some of the techniques for dealing with unruly data. Master lot testing for a serum protein to be used on an automated immunoanalyzer provides a good example of the application of designed experiments. The experiment discussed in detail here was designed and analyzed

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  • Fluorescent Proteins I
    On the other hand, since tFl can be measured independent from the fluorescence intensity, FP mutants with prolonged tFl can be found as long as some visible protein fluorescence is detected.
  • Principles of Fluorescence Spectroscopy
    Protein Fluorescence .
  • Fluorescent Proteins II
    The rigid tertiary structure of wtGFP is responsible for the significant protein fluorescence emission (FA ¼ 0.78 [57], FB ¼ 0.79 [48]), which is also very .
  • Advanced Fluorescence Reporters in Chemistry and Biology I
    Maddalo SL, Zimmer M (2006) The role of the protein matrix in green fluorescent protein fluorescence .
  • Handbook Of Biological Confocal Microscopy
    (A) Percentage of single- protein fluorescence recovery versus the illumination time at 720nm for different excitation powers: 3.34mW (solid circles), 4.34mW (solid squares), 5mW (open squares), and 5.7mW (open circles).
  • Topics in Fluorescence Spectroscopy
    simultaneously, Duggan and Udenfriend(8) and Shore and Pardee(9) reported the results of their investigations of protein fluorescence .
  • Topics in Fluorescence Spectroscopy
    Volume 6 Protein Fluorescence .
  • Green Fluorescent Protein
    ...59 Phenol-chloroform extractions, 66 Phogrin-cameleon construct domain structure, 256f Phosphorescence energy-level diagram, 94 Phosphoribosylanthranilate isomerase circular permutation, 33 Phosphorylated synthetic sal/linkers ligation, 62 Phosphorylation GFP, 283f Phycobilisome FRET, 113 Phycobilisome-bound GFP fusion protein fluorescence and rotational diffusion characteristics...
  • High Content Screening
    Intrinsic protein fluorescence and .