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  • Biotechnology for Fuels and Chemicals
    The conidia were then added to the medium for conidial swelling and incubated for 6 h using a rotary shaker (TAITEC NR-30, Koshigaya, Japan) at 28°C.
  • Microarrays
    Place membrane on rotary shaker set at 3 for 30 min. 9.
  • Applied Biochemistry and Biotecnology
    These flasks were seeded with 0.1 mL from the 24 h-old culture containing 2.6 x 109 cells/mL, and were incubated for 48 h on a rotary shaker at 37°C and 150 revImine After a 48-h fermentation, the contents...
  • Protocols Volume 2
    The contents are agitated on a rotary shaker at 225 rpm and 30°C for 3 h, the cells are then diluted at a 1:10 ratio with freshYM and plated on solidYM medium con- taining 100 mg/L streptomycin and 50 mg/L kanamycin.
  • Protocols
    Keep at 21qC on a rotary shaker (120 rotations/min) for at least 120 min to label the entire endocytic pathway (see Note 16).
  • Microbial Processes and Products
    Incubate the bacteria in a rotary shaker (250 rpm at 30°C) until OD540 reaches a value of 0.14 (diluted 1/10, absolute value = 1.4).
  • Plant Cell Culture Protocols
    A large walk-in growth room in which can be placed not only shelves, but also rotary shakers , bioreactors, and so on, reduces flexibility, but is generally more economical.
  • Transgenic Plants
    After the 70% ethanol is removed, 40 mL of 2.5% sodium hypochlorite containing one drop of Tween 20 is added, and the tube is placed on a rotary shaker and shaken gently for 15 min. 4.
  • Chemoenzymatic synthesis of chiral carboxylic acids via nitriles
    The two vials (blank with- out substrate) were shaken at 30 ◦ C on a rotary shaker at 180 rpm.
  • Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals
    Hydrolysis was performed at 45°C on a rotary shaker at 150 rpm with peri- odic sampling for sugar analysis.
  • Protocol for Somatic Embryogenesis in Woody Plants
    Place the flask on a rotary shaker with shaking at 150 rpm in the light with a photoperiod of 16 h (40 Pmol m-2 s-1 cool white fluorescent lamps).
  • Carbohydrate Biotechnology Protocols
    A rotary shaker is used to study the fermentation process at the shaken-flask scale, and a 3-dL stirred-tank fermenter STF (Applikon, Schiedam, NL) is used for performing the laboratory-scale experimentation.
  • Plant Cell and Tissue Culture
    with 2 mg/L 2,4-D and 0.2 mg/L kinetin, and incubated on a rotary shaker at 80 rpm at 25OC.
  • Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals
    Cultures were done in 500-mL conical flasks and grown at 29°C on a rotary shaker (150 rpm).