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  • Biotechnology for Fuels and Chemicals
    The conidia were then added to the medium for conidial swelling and incubated for 6 h using a rotary shaker (TAITEC NR-30, Koshigaya, Japan) at 28°C.
  • Microarrays
    Place membrane on rotary shaker set at 3 for 30 min. 9.
  • Applied Biochemistry and Biotecnology
    These flasks were seeded with 0.1 mL from the 24 h-old culture containing 2.6 x 109 cells/mL, and were incubated for 48 h on a rotary shaker at 37°C and 150 revImine After a 48-h fermentation, the contents …
  • Protocols Volume 2
    The contents are agitated on a rotary shaker at 225 rpm and 30°C for 3 h, the cells are then diluted at a 1:10 ratio with freshYM and plated on solidYM medium con- taining 100 mg/L streptomycin and 50 mg/L kanamycin.
  • Protocols
    Keep at 21qC on a rotary shaker (120 rotations/min) for at least 120 min to label the entire endocytic pathway (see Note 16).
  • Microbial Processes and Products
    Incubate the bacteria in a rotary shaker (250 rpm at 30°C) until OD540 reaches a value of 0.14 (diluted 1/10, absolute value = 1.4).
  • Plant Cell Culture Protocols
    A large walk-in growth room in which can be placed not only shelves, but also rotary shakers , bioreactors, and so on, reduces flexibility, but is generally more economical.
  • Handbook for Azospirillum
    Usually, rhizosphere soil (10 g) is also homogenized for 30–60 min in 90 mL of saline solution (eg. salts of NFb medium) or sucrose solution (4 %), using a rotary shaker at 150 rpm.
  • Advances in Applied Biotechnology
    After cultivation at 37 °C on a rotary shaker (180 r/min) to the late logarithmic phase, the cultures were transferred into the fresh challenging medium to cultivate for 2–3 times under the same conditions.
  • Progress in Environmental Science and Engineering
    The cultures were incubated at 30℃ in a rotary shaker at 150r/min for 7d.
  • Transgenic Plants
    After the 70% ethanol is removed, 40 mL of 2.5% sodium hypochlorite containing one drop of Tween 20 is added, and the tube is placed on a rotary shaker and shaken gently for 15 min. 4.
  • Materials for Environmental Protection and Energy Application
    The flasks were kept in a rotary shaker with 200 rpm for 48 hours at 22 °C.
  • Chemoenzymatic synthesis of chiral carboxylic acids via nitriles
    The two vials (blank with- out substrate) were shaken at 30 ◦ C on a rotary shaker at 180 rpm.
  • Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals
    Hydrolysis was performed at 45°C on a rotary shaker at 150 rpm with peri- odic sampling for sugar analysis.
  • Protocol for Somatic Embryogenesis in Woody Plants
    Place the flask on a rotary shaker with shaking at 150 rpm in the light with a photoperiod of 16 h (40 Pmol m-2 s-1 cool white fluorescent lamps).