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...such as veterinary drugs and pesticides and pathogenic bacteria and their toxins in food products. To detect E. coli O157:H7 in. foods, chemist Shu-I Tu and. microbiologist Marsha Golden use. immunomagnetic capture and. time-resolved fluorescence. (D009-1). Shu-I Tu, research leader...

...range for analyte detection. Figure 2. The time-resolved fluorescence experiment. A light flash occurs at time zero, and fast fluorescence emission occurs early on. The sample-delayed emission occurs and may proceed up to 1 millisecond (depending on the sample). Data collection proceeds after 50...

...tests and not as confirmatory tests. Time-Resolved Fluorescence. Although a seldom-used older technology, time-resolved fluorescence (TRF) employs the basic analyte sandwich capture format. The strength of TRF over conventional ELISAs is increased sensitivity and the potential for multiplexing...

R &D Digest: The monthly review of new technologies and medical device innovations. A fiber-optic probe and a special interferometer could spot early signs of cancer faster than current methods and thus reduce the need for biopsies. The technology, called angle-resolved low-coherence interferometry...

times (200 nm and >500 ns, respectively). Time-resolved fluorescence takes advantage of these long decay times and large Stokes' shifts, making them qualifiers for the fluorescent signal to be recorded. Thus, any short-lived fluorescent background signal or scattered excitation radiation can...

...with wash steps, although other methods (e.g., time-resolved fluorescence) are also available. By attaching capture antibody to the surface of a piezofilm, an assay can discriminate between the labeled antibody bound to the surface of the film and the antibody in solution without conducting a separation...

...fluorophores give a sample emission that is about 1 picosecond after excitation while in time-resolved fluorescence the emission occurs after a microsecond lag. Good Manufacturing Practices (GMPs). Regulations promulgated by the Food and Drug Administration governing the manufacture of drugs (Ref...

...are illustrated in Figure 4. Excellent agreement between. SHG) and a spectral measurement of the output. In the case. expected and measured values is evident. of Frequency Resolved Optical Gating (FROG), the linear. filtering consists of generating two time-shifted replicas of. the original pulse. 10...

...says Mimms. Currently, "fluorophore labels dominate all microformats in DNA testing," he says. But manufacturers are also experimenting with the use of time-resolved fluorescence, dendrimers, new uses of iodine radioisotopes, and chemical locks derived from improved washing techniques. In the very...

...are the data are best left alone. Use a More-Complex Model. Although it is sometimes of value, use of a more-complex model requires further data collection. Time and resource availability may be the deciding factors here. Assessing Data Adequacy Once the lack-of-fit issue is resolved, the experimenters may...

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  • Principles of Fluorescence Spectroscopy
    Time resolved fluorescence spectroscopy of cyanine dyes.
  • Reviews in Fluorescence 2005
    In recent years, time resolved fluorescence spectroscopy has yielded a wealth of information on the polarity and solvation dynamics in many organized assemblies.^"^ The fluorescence data is supplemented by large scale computer simulations on dielectric constant and dynamics in organized assemblies...
  • Who’s Who in Fluorescence 2005
    Development and implementation of new data analysis methods for steady state and time resolved fluorescence data.
  • Chlorophyll a Fluorescence
    Time resolved fluorescence spectra at 77 K of Acaryochloris marina .
  • Standardization and Quality Assurance in Fluorescence Measurements I
    Fig. 7 A FLIM image is an array of pixels, each pixel containing a time resolved fluores- cence measurement .
  • Lanthanide Luminescence
    Mathis G, Socquet F, Viguier M, Darbouret B (1997) Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence : principles and specific advantages for tumor markers.
  • Topics in Fluorescence Spectroscopy
    R. B. Cundall and R. E. Dale (eds.), Time Resolved Fluorescence Spectroscopy in Bio- chemistry and Biology, Plenum, New York (1983).
  • Topics in Fluorescence Spectroscopy
    J. R. Lakowicz, I. Gryczynski, Z. Gryczynski, E. Danielsen, and M. J. Wirth, Time resolved fluorescence intensity and anisotropy decays of 2,5-diphenyloxazole by two-photon excitation and frequency-domain fluorometry, J. Phys. Chem. 96, 3000–3006 (1992).
  • Topics in Fluorescence Spectroscopy
    The rotational correlation time, , of a tryptophan residue can be determined from time resolved fluorescence anisotropy measurements.30 values are very useful due to their relationship to protein structure.
  • Biophysical Techniques in Photosynthesis
    Optical methods and phenomena that are discussed include linear and circular dichroism, time resolved fluorescence and absorbance measurements, including their data analy- sis, vibrational spectroscopy (infrared and resonance Raman) and specialized techniques, such as photon echo, hole burning, Stark and photoacoustic spectroscopy.
  • Photosynthesis. Energy from the Sun
    Fig. 2 Species associated emission spectra (SAES) from the modeling of the time resolved fluorescence data of core (left) and intact (right) PSI particles .
  • Topics in Fluorescence Spectroscopy
    Mathis, G., Socquet, F., Viguier, M., and Darbouret, B. Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence : Principles and specific advantages for tumor markers.
  • Anoxygenic Photosynthetic Bacteria
    ...constant (9 ps) deduced from the analysis of the fluorescence data is considerably longer than what has been concluded for Rb. sphaeroides from transient absorption (van Grondelle et al., 1987; Zhang et al., 1992b) and time resolved fluorescence measurements at 77 K...
  • Handbook Of Biological Confocal Microscopy
    Early applications of two-photon microscopy (2PM) to the study of dynamic biochemical processes in living cells demon- strated some of the advantages for quantitative three-dimensional (3D) and four-dimensional (4D) (space and time ) resolved fluo- rescence microscopy (see below).
  • Reviews in Fluorescence 2007
    Johansson, Aggregation of an α- helical transmembrane peptide in lipid phases, studied by time resolved fluorescence spec- troscopy, J. Phys. Chem.

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