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Supplier: SPIE - Education
Description: is to present the factors that limit the accuracy, resolution, and reproducibility of microscopic imaging of biological objects. The discussion will focus on two methods of 3D optical imaging: confocal microscopy and two-photon microscopy. The course will recapitulate the
- Modality: On-site / In Plant
- Industry: Life Sciences
- Technology / Subject: Bioengineering / Biomedical, Design / Engineering Methods (ESDU, DFx, etc.), Photonics / Optics
Supplier: Newport Corporation
Description: as dichroic mirrors in pump probe laser applications. The mirrors have a 10 arc minute wedge angle to suppress interference fringes for the transmitted beam. Note that performance outside of the specified wavelength range cannot be guaranteed. Two Photon Microscopy Applications
- Incidence: 45º Incidence
- Diameter/Width: 1 inch
- Wavelength Range: 670 to 1340 nm
- Mirror Materials: Fused Silica
Description: lenses, and vertical displacement microlenses. A discussion of varifocus elements demonstrated in widefield microscopes, confocal microscopes, two-photon microscopes, and optical coherence tomography is included. This book serves as a course module in electrical engineering and physics, whileShow More
Description: techniques 61 3.2.5. Nonlinear optics in 1D PBGs 62 3.3. Higher dimensions 63 3.3.1. Vector wave equations 64 3.3.2. Two dimensions 65 3.3.3. Dielectric fluctuations 68 3.3.4. Band structure 69 3.3.5. Band eigenfunction symmetry and uncoupled modes 71 3.3.6. Three dimensions 73 3.4. SummaryShow More
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Two-photon microscopy with diffractive optical elements and spatial light modulators
Two-photon microscopy is often performed at slow frame rates due to the need to serially scan all points in a field of view with a single laser beam. To overcome this problem, we have developed two optical methods that split and multiplex a laser beam across the sample.
Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing
The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based
Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection
provide optical sectioning required for 3-D resolution. On the other hand, techniques that do provide 3-D resolution, such as confocal or two-photon microscopy, typically offer only limited fields of view and penetration depths.1 Macroscopic imaging with such techniques is quite laborious
Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
We propose two-photon excited fluorescence (TPEF) microscopy employing a novel phase modulation technique of ultra-broadband laser pulses, which allows the relative excitation of an individual fluorophore with respect to other fluorophores. This technique is based on the generation
Microprisms are key to brain exploration
An opening in the mouse skull, or cranial window, enables imaging the neurons in the living brain using two-photon microscopy. But using this approach, one can generally only image the most superficial layers of the cortex. To overcome this, Andermann et al. use microprisms. They inserted 1-mm
Quantitative morphometric measurements using site selective image cytometry of intact tissue
, high-resolution three-dimensional assays based on two-photon microscopy can be performed only in these regions of interest. We further show that three-dimensional morphology extraction algorithms can be used to analyse the resultant high-resolution two-photon image stacks providing information
Multimodal Nonlinear Optical (NLO) Imaging
NLO imaging modalities are two- and three-photon fluorescence (2P & 3P), second- and third-harmonic generation (SHG & THG), and coherent Raman scattering (CRS) in the form of either Coherent Anti-Stokes Raman Scattering (CARS) or Stimulated Raman Scattering (SRS).
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Handbook Of Biological Confocal Microscopy
So, P.T.C., French, T., Yu, W.M., Berland, K.M., Dong, C.Y., and Gratton, E., 1996, Two photon microscopy : Time-resolved and intensity imaging, In: Fluorescence Imaging Spectroscopy and Microscopy (X.F. Wang and B. Herman, eds.), John Wiley …
Advances in Intravital Microscopy
Two Photon microscopy FITC .
… 267 Tumor-draining, 267–271, 282 Two-photon (2P), 267–271, 273, 275, 277, 279, 281–282 Two-photon imaging advantages, 3, 9–11 requirements, 1, 5–9 Two-photon microscope construction, 12–16 dispersion compensation, 19–20 optimization, 16–21 software, 15–18 Two photon microscopy , 72, 74–75, 209–210 advantages …
foci were simultaneously visualized by using two photon microscopy .
Fundamentals of Fluorescence Microscopy
Two photon microscopy is a suitable alternative for laser scanning fluorescence microscopy, which is due to its intrinsic intensity-squared behavior (see Chap. 8).
Fluorescent Methods to Study Biological Membranes
2008) Pig skin structure and transdermal delivery of liposomes: a two photon microscopy study.
Second, we developed a depth resolved widefield two photon endomicroscope for the medical diagnosis based on the temporally focused widefield two photon microscopy .
New Techniques in Systems Neuroscience
Further, dMd stimulation can be coupled with two photon microscopy to read out activity patterns from a large number of cells as opposed to one or a few cells accessed by electrical recordings (Sect. 9.3.11). all these studies demonstrate the …
The design of the two photon microscopy is described in the INTRODUCTION .