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Supplier: SPIE - Education
Description: emphasis on high resolution two-photon 4Pi-microscopy and real-time direct view multifocal multiphoton microscopy (MMM).
- Modality: On-site / In Plant
- Industry: Life Sciences
- Technology / Subject: Bioengineering / Biomedical, Photonics / Optics
Description: lenses, and vertical displacement microlenses. A discussion of varifocus elements demonstrated in widefield microscopes, confocal microscopes, two-photon microscopes, and optical coherence tomography is included. This book serves as a course module in electrical engineering and physics, while alsoShow More
Supplier: Newport Corporation
Description: as dichroic mirrors in pump probe laser applications. The mirrors have a 10 arc minute wedge angle to suppress interference fringes for the transmitted beam. Note that performance outside of the specified wavelength range cannot be guaranteed. Two Photon Microscopy Applications In two photon microscopy
- Mirror Types: Laser Mirror
- Wavelength Range: 670 to 1340 nm
- Diameter/Width: 1 inch
- Mirror Shape: Round
Supplier: Thorlabs, Inc.
Description: a complete product line ranging from objectives to anti-vibration work stations allowing you to create the ideal system to meet your imaging needs and your budget. Multiphoton excitation occurs when two (or more) photons, whose summed energy is sufficient to excite a fluorophore, arrive
- Application: Biological / Life Science
- Grade: Benchtop
- Microscope Type: Other
- Digital Display: Yes
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Two-photon microscopy with diffractive optical elements and spatial light modulators
Two-photon microscopy is often performed at slow frame rates due to the need to serially scan all points in a field of view with a single laser beam. To overcome this problem, we have developed two optical methods that split and multiplex a laser beam across the sample.
Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing
The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based
Calcium imaging of inner ear hair cells within the cochlear epithelium of mice using two-photon microscopy
Mice are an excellent model for studying mammalian hearing. and transgenic mouse models of human hearing, loss are commonly. available. However, the mouse cochlea is substantially smaller. than other animal models routinely used to study cochlear physiology. This makes study of their hair cells
Two-Photon Excited Fluorescence Microscopy
Several laser-scanning microscopy techniques have been developed in the last twenty years based on non-linear optical phenomena.1 This has led to a variety of powerful imaging tools, such as two-photon excited fluorescence microscopy (TPM),2 second harmonic generation (SHG) microscopy
Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection
provide optical sectioning required for 3-D resolution. On the other hand, techniques that do provide 3-D resolution, such as confocal or two-photon microscopy, typically offer only limited fields of view and penetration depths.1 Macroscopic imaging with such techniques is quite laborious
Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
We propose two-photon excited fluorescence (TPEF) microscopy employing a novel phase modulation technique of ultra-broadband laser pulses, which allows the relative excitation of an individual fluorophore with respect to other fluorophores. This technique is based on the generation
Microprisms are key to brain exploration
An opening in the mouse skull, or cranial window, enables imaging the neurons in the living brain using two-photon microscopy. But using this approach, one can generally only image the most superficial layers of the cortex. To overcome this, Andermann et al. use microprisms. They inserted 1-mm
Quantitative morphometric measurements using site selective image cytometry of intact tissue
, high-resolution three-dimensional assays based on two-photon microscopy can be performed only in these regions of interest. We further show that three-dimensional morphology extraction algorithms can be used to analyse the resultant high-resolution two-photon image stacks providing information
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Handbook Of Biological Confocal Microscopy
So, P.T.C., French, T., Yu, W.M., Berland, K.M., Dong, C.Y., and Gratton, E., 1996, Two photon microscopy : Time-resolved and intensity imaging, In: Fluorescence Imaging Spectroscopy and Microscopy (X.F. Wang and B. Herman, eds.), John Wiley …
Advances in Intravital Microscopy
Two Photon microscopy FITC .
… 267 Tumor-draining, 267–271, 282 Two-photon (2P), 267–271, 273, 275, 277, 279, 281–282 Two-photon imaging advantages, 3, 9–11 requirements, 1, 5–9 Two-photon microscope construction, 12–16 dispersion compensation, 19–20 optimization, 16–21 software, 15–18 Two photon microscopy , 72, 74–75, 209–210 advantages …
foci were simultaneously visualized by using two photon microscopy .
Fundamentals of Fluorescence Microscopy
Two photon microscopy is a suitable alternative for laser scanning fluorescence microscopy, which is due to its intrinsic intensity-squared behavior (see Chap. 8).
Fluorescent Methods to Study Biological Membranes
2008) Pig skin structure and transdermal delivery of liposomes: a two photon microscopy study.
Second, we developed a depth resolved widefield two photon endomicroscope for the medical diagnosis based on the temporally focused widefield two photon microscopy .
New Techniques in Systems Neuroscience
Further, dMd stimulation can be coupled with two photon microscopy to read out activity patterns from a large number of cells as opposed to one or a few cells accessed by electrical recordings (Sect. 9.3.11). all these studies demonstrate the …