From CILAS

Different characterization principles are used to measure the size of polymeric microspheres. Let us first discuss the microscopic techniques that are based on the direct observation of a sample. One method is electronic microscopy that is highly reliable but very expensive. It requires time and specific know-how in the preparation of the sample, thus making the result not immediately accessible.
Therefore microscopy for the characterization of size of the particles is not easily applicable for analyses in real time.

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