From Sherry Laboratories

Does it really matter if there are air bubbles in these 40mL VOA vials? You may save yourself time and expense by understanding the Zero Headspace procedure, and how to maintain the integrity of your aqueous samples for volatile organic analysis from time of collection until they arrive at the lab. The presence of headspace and air bubbles in volatile organic analysis sampling vials lowers the actual aqueous concentration of these compounds due to the partitioning of solutes into the gaseous phase. This could make the sample invalid for analysis.

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