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UV-VIS Spectroscopy of Tannins and Phenols in Red Wine
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UV-VIS Spectroscopy of Tannins and Phenols in Red Wine
 
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UV-VIS Spectroscopy of Tannins and Phenols in Red Wine
From ALine, Inc.
 

 
Measurement of wine color and tannins involves spectroscopic analysis in the UV and visible region. The test protocols are complex and are hampered by the need make multiple wavelength measurements. A typical spectrophotometer can only make one wavelength measurement at a time. In addition, the assessment of total phenols requires measurement of the sample at 280 nm requiring the use of an expensive, difficult to clean and easy to break 1 mm path length quartz cuvette. Even with a 1mm pathlength, most red wines still require 5 to 10 fold dilution in order to keep the absorption at 280 nm near 2.0 OD, further complicating the test protocol. In this application note, we report the performance of a new short path cuvette, the SpecVette™ with an Ocean Optics HR4000 spectrometer using a balanced Deuterium- Halogen light source to measure the absorbance of undiluted red wine samples from 250 to 650 nm. A DOS routine was written to automatically collect and transfer ten sets of data from the SpectraSuite software into and Excel™ spreadsheet. Data was collected from either ten different cuvettes (inter-cuvette measurements) or from a single cuvette that was repeatedly measured after removal and re-insertion into the cuvette holder (intra-cuvette measurements). The ten sets of data transferred into the spreadsheet were averaged at each 0.25 nm interval across the entire spectrum and the standard deviation and % error calculated. The standard deviation of the measurement at each 0.25 nm from 250 to 600 nm was plotted. The % error across the same range was plotted separately below the spectrum.

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Product Announcements
ALine, Inc. - ALine Short Path Length, UV Transparent Cuvettes
Short Path Length, UV Transparent Cuvettes perfect for Spectroscopy of Optically Opaque Tannins and Phenols: Easy- fill SpecVette™ permits dilution-free measurement of red wine samples in a... (read more)
ALine, Inc. - Microfluidic Cuvettes - Try and Buy Promo
Microfluidic Cuvettes for Fluorescence & Absorbance Try our sample kits: FluoroVettes(TM) for micro volume fluorescence in either single sample or flowcell format, and short pathlength SpecVettes... (read more)
StellarNet, Inc. - StellarNet InGaAs Extended Range Spectrometers
StellarNet has announced the NIRX-SR series of spectrometers for the 0.9-2.3μm wavelength range with a 512 element InGaAs Photo Diode Array and high speed USB-2.0 interface. The extended range... (read more)
optek-Danulat, Inc. - UV Sensor Calibration Accessories
Once only available for calibration of spectrophotometers, a complete NIST-traceable calibration package is now available for the process line. Optek delivers superior measurement confidence with the... (read more)
StellarNet, Inc. - Dual Detector Spectrometers
The StellarNet DUAL-DSR spectrometers are fiber optically coupled instruments that cover a "Super Range" from 0.2-2.3um for spectroscopy measurements on a PC via USB-2 interface. The spectrograph... (read more)
Thermo Scientific - Water Analysis/Orion Products - Orion AQUAfast® IV Colorimeter
The Orion AQUAfast IV AQ4000 multi-wavelength colorimeter stores up to 189 pre-programmed methods. As new tet are available, a simple upload procedure updates the colrimeter with the most current... (read more)
Shimadzu Scientific Instruments, Inc. - UV-1800 Spectrophotometer
Designed in accordance with governing Pharmacopoeia, the new UV-1800 UV-VIS spectrophotometer achieves a resolution of 1 nm, the highest in its class, in a compact design. Offering an array of... (read more)
StellarNet, Inc. - Fluorometer
StellarNet has announced the CXR-FS, a low cost system for fluorescence spectroscopy. The components include a miniature fiber optically coupled, thermo electrically cooled (TEC) UV-VIS spectrometer... (read more)
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Topics of Interest
Bio Micro Solutions Newsletter - August 27, 2007 Short Path Length, UV Transparent Cuvettes perfect for Spectroscopy of Optically Opaque Tannins and Phenols Easy- fill SpecVette™ permits... (Read More)
To determine APHA color on-line for process control or in the lab for product release. Aproach: An Optical Solutions' PS-2-UV/VIS diode array spectrophotometer and HeaterCell™ fiber optic... (Read More)
Purpose: Measurement of water in a multiple alcohol and brine solution with a ChemView® photometer. Experimental: PS-2E fiber optic diode array NIR spectrometer, 2 m low-OH optical fibers,... (Read More)
SPECTROPHOTOMETRY A spectrophotometer measures the relative amounts of light energy passed through a substance that is absorbed or transmitted. We will use this instrument to determine how much... (Read More)
Challenge: Can quantitative analysis be done with a multiple wavelength filter photometer? Background: The spectra of this 3-component mixture are complex. Initial visual inspection suggests that a... (Read More)
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