Secondary Structure Analysis of Proteins Using IR Imaging

The IMV-4000 enables infrared imaging by a figure of two or more faster than using a conventional single element detector. A technique that quickly analyzes the results is also desirable. JASCO has proposed an analysis of imaging data with a variety of multivariate analysis techniques to accelerate the processing of infrared imaging data. For example, we have made it possible to easily create a visualization of the distribution map for the various components of multilayer film by analyzing imaging data using principle component analysis (PCA). We have also created a model that performs secondary structure estimation (SSE) on proteins based on IR spectra by principal components regression (PCR) and partial least squares (PLS) regression. In the secondary structure estimation of proteins, crystallizing the protein and performing x-ray structural analysis and measuring aqueous solutions by NMR or CD spectra are all widely used. However, the estimation of the secondary structure of proteins while preserving the tissue structure in multicomponent systems such as biological structures is extremely difficult with those techniques.