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Principles and Practical Applications of Shimadzu's ELSD-LT II Evaporative Light Scattering Detector

From Shimadzu Scientific Instruments, Inc.
 

 
In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambient temperature, their sensitivity is low compared to that of UV detectors, and they are prone to giving negative peaks, which make quantitative analysis difficult.
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