From New Directions in Bioprocess Modeling and Control: Maximizing Process Analytical Technology Benefits

6-1 Introduction

Recently, a fed-batch fermentation simulation was developed by the Department of Chemical and Environmental Engineering at the Illinois Institute of Technology and made available to the public as a test bed for control and optimization studies. This model is the basis of a virtual penicillin production plant developed to run on a distributed control system (DCS). Elements of this test-bed model were incorporated into a fed-batch model of human epidermal growth factor (hEGF) production by recombinant bacteria; and, into a batch model of simultaneous saccharification and fermentation (SSF) by baker's yeast.

In this chapter, a model of a cell culture that divides the cell mass into four units or pools is presented to demonstrate the increase in the complexity of mass balances when a model considers cell structure. Section 6-2 begins our discussion of virtual bioreactors by pinpointing our location on a map of model types. Section 6-3 shows how mass, energy, and component balances are used to create a virtual plant. Section 6-4 gives the heat of reaction used in energy balances in all three simulations. Section 6-5 shows how pH can be calculated in a fermentation broth by a charge balance on weak acids and weak bases. Section 6-6 provides the values of the constants and initial values of the variables used in all three virtual plants. Section 6-7 on kinetics presents the calculations of the specific growth rate of each culture and shows how product formation is correlated to specific growth rate. Section 6-8 discusses...

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Topics of Interest

Robert L. Heider, Adjunct Professor Chemical Engineering Department Washington University St. Louis,, MO Mike May, Process Engineer Haldor Topsoe, Inc. Pasadena,, TX Ashish Mehta, Principal Software...

Overview James N. Tilton, Ph.D., P.E. Principal Consultant, Process Engineering, E. I. du Pont de Nemours & Co.; Member, American Institute of Chemical Engineers; Registered Professional Engineer...

6-1 SCOPE In Secs. 6-2 and 6-3, we develop rigorous relations for the enthalpies, entropies, Helmholtz and Gibbs energies, and fugacities that are used with equations of state (EoS) and ideal gas...

Overview One situation seen in all types of production is the splitting and merging of batches in a work center. This is common in many industries, such as in mixing of batches in brewing and...

6.1 Introduction: “To Batch or Not to Batch” The automation of processes had its theoretical and practical application origins in the continuous petrochemical industries, starting in the...