Volatiles and Class II Type A2 BSCs
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BSC MythBusters - Volatiles & Class II Type A2 BSCs: How much is safe?
Join Dr. Kara Held Ph.D., Baker's Science Director, for this session on Volatiles and Class II Type A2 BSCs.
Volatile chemicals, while typically used in a fume hood, sometimes need to be used in conjunction with biological experiments, requiring the use of volatile chemicals within a biosafety cabinet (BSC).
While the safest option often mentioned is a 100% exhausted Class II Type B2 cabinet, this is not always possible. International safety standards have recommended “minute” amounts of volatiles can safely be used in a Class II Type A2, but what constitutes “minute” can be left up for interpretation.
At Baker, we have derived an equation to calculate the internal concentration, as well as the time required for the evacuation of any volatile chemical, shown both by calculation and experimentation.
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ABOUT BAKER Since 1949, The Baker Company has been an industry pioneer in the field of biological safety with an unparalleled passion for helping its customers advance science, discovery and clinical care. The company was first established in response to a growing need to protect hospital workers from infectious diseases and the dangerous process of drug preparation. As solutions for air containment and contamination control have evolved over the years, Baker has been at the forefront of design and innovation, insisting on taking no shortcuts and applying no minimum standards to the solutions that they supply. Its Baker Ruskinn division is one of the world's leading suppliers of gas-controlled, anaerobic and modified atmosphere workstations for use in medical microbiology, cell biology, cancer research, molecular medicine and stem cell laboratories.