DENARASE Enzyme Used in Large-Scale Bioprocesses

Product Announcement from Sartorius Group

DENARASE Enzyme Used in Large-Scale Bioprocesses-Image

DENARASE is a highly active endonuclease derived from Serratia marcescens. This enzyme is well known for its combined RNase and DNase activity and is therefore used in many large-scale bioprocesses.

DNA can cause high viscosity in bioprocesses and often interacts with the target product, resulting in lower recovery rates. DENARASE reduces the DNA level at an early stage of the process and improves recovery rates.  Successful applications of DENARASE include the purification of vaccines, proteins expressed in inclusion bodies and monoclonal antibodies.


  • Biotech products must be free of host cell DNA larger than 100 bases. DENARASE leaves DNA chains of 3 – 8 bases and can be used as DNA removal technology.
  • High DNA concentrations cause high viscosities which makes filtration and chromatography difficult. DENARASE helps to reduce the viscosity and to improve the process economics.
  • DNA can stick to the target molecule like Mab’s and Viruses. Coverage of binding groups can lead to lower recovery rates for chromatography steps.
  • DENARASE can prevent cell clumping in bioreactors 

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