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  • Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
    . We also demonstrate yielded equal excitation rates in the simultaneous excitation. By the selective excitation of a donor fluorescent protein, fluorescence resonance energy transfer imaging is also achieved.
  • Medical Device Link . Measuring microsphere binding capacity
    these magnetic microspheres preconjugated with some type of generic binding protein. A common choice is streptavidin (from , or produced recombinantly), which is similar to the avian egg protein avidin. Streptavidin interacts strongly with the molecule biotin, and so by biotinylating the ligand to be coupled
  • Medical Device Link .
    Point-of-care diagnostics (POCT) promises reduced costs, small sample volumes, ease of use and the possibility of the continuous monitoring of patients. Simultaneous measurement of different proteins is necessary to cover the highly dynamic concentration range that is relevant for safe diagnostics
  • Medical Device Link . Formal experimental design and analysis for immunochemical product development
    . As the example proceeds, it will look at some of the techniques for dealing with unruly data. Master lot testing for a serum protein to be used on an automated immunoanalyzer provides a good example of the application of designed experiments. The experiment discussed in detail here was designed and analyzed
  • Medical Device Link .
    of medical diagnostics are still conducted in clinical labs using large equipment. This article examines current commercial systems and their emerging alternatives. One technology in particular high-throughput multiantigen fluorescence immunoassays in polydimethylsiloxane (PDMS) microfluidic chips
  • Medical Device Link .
    dye from Amersham Biosciences AB (Uppsala, Sweden); specifically, the monofunctional Cy5 fluorophore whose absorption maximum is near 650 nm and fluorescence maximum is near 670 nm. When the fluorescent Cy5 was conjugated to a dextran chain by means of the technology developed by Amdex a/s (Jyllinge

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  • Fluorescent Proteins I
    On the other hand, since tFl can be measured independent from the fluorescence intensity, FP mutants with prolonged tFl can be found as long as some visible protein fluorescence is detected.
  • Fluorescent Proteins II
    The rigid tertiary structure of wtGFP is responsible for the significant protein fluorescence emission (FA ¼ 0.78 [57], FB ¼ 0.79 [48]), which is also very .
  • Principles of Fluorescence Spectroscopy
    Protein Fluorescence .
  • Advanced Fluorescence Reporters in Chemistry and Biology I
    2006) The role of the protein matrix in green fluorescent protein fluorescence .
  • Handbook Of Biological Confocal Microscopy
    (A) Percentage of single- protein fluorescence recovery versus the illumination time at 720nm for different excitation powers: 3.34mW (solid circles), 4.34mW (solid squares), 5mW (open squares), and 5.7mW (open circles).
  • High Content Screening
    Intrinsic protein fluorescence and .
  • Green Fluorescent Protein
    … 59 Phenol-chloroform extractions, 66 Phogrin-cameleon construct domain structure, 256f Phosphorescence energy-level diagram, 94 Phosphoribosylanthranilate isomerase circular permutation, 33 Phosphorylated synthetic sal/linkers ligation, 62 Phosphorylation GFP, 283f Phycobilisome FRET, 113 Phycobilisome-bound GFP fusion protein fluorescence and rotational diffusion characteristics …
  • Topics in Fluorescence Spectroscopy
    simultaneously, Duggan and Udenfriend(8) and Shore and Pardee(9) reported the results of their investigations of protein fluorescence .
  • Topics in Fluorescence Spectroscopy
    Volume 6 Protein Fluorescence .
  • Fluorescent Methods for Molecular Motors
    Unfortunately, ADP binding to actin.S1 does not normally result in a fluorescence signal, either using intrinsic protein fluorescence or the pyrene label.