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Understanding Microscopes

From Edmund Optics Inc.
 

 

Compound microscopes differ from simple magnifiers in that there are two separate magnification steps that occur instead of one. The objective lens is nearest the subject under observation and provides a magnified real image. The eyepiece magnifies the real image provided by the objective and yields a virtual image appropriate for the human eye. Remember that the eye has its own lens, which relays virtual images onto the retina.

Microscopes have been standardized over time to simplify design and manufacture. Most microscopes employ the Deutsche Industrie Norm, or DIN standard configuration, while the Japanese Standard (JIS) is less commonly used. DIN microscopes begin with an object-to-image distance of 195mm, and then fix the object distance (with respect to the rear shoulder of the objective) at 45mm. The remaining 150mm distance to the eyepiece field lens sets the internal real image position, defined as 10mm from the end of the mechanical tube. Objective lens thread is the same for DIN and JIS, that is 0.7965" (20.1mm) diameter, 36 TPI, 55° Whitworth.


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