Chromatography Roundup

Also to be introduced this year, Paradis notes, are production-scale (up to 200-mm-diameter) supercritical fluid chromatography (SFC) systems. Pall is also seeing customers expand applications of its Mustang membrane chromatography line. "Manufacturers are starting to use membrane adsorbers not only for polishing downstream, but also further upstream in the capture steps," Jones says. Membrane adsorbers are being used to protect high-value chromatography media by removing increasing amounts of endotoxins, viral contaminants, and chromosomal DNA debris that may result from the higher protein titers being seen upstream. (Goettingen, Germany) continues to be at the forefront of membrane chromatography with its Sartobind line. The company has increased the loading capacity of Sartobind Q for MAb polishing — 10.9 kg MAb per liter sorbent, says Jeff Mora, product manager for membrane chromatography. This allows just one to two liters of Sartobind to clear contaminants and viruses from a 15,000-liter MAb production run. A 1- or 2-liter membrane is approximately 200 times smaller than a column for the same unit operation, and the membrane can process five to 10 times faster, Mora says. The smaller sorbent volume means 95% less buffer consumption, and thus cost savings. As with other disposables, Sartobind SingleSep Q capsules are disposable and carry no cleaning validation or initial hardware costs, says Mora. (Kyoto, Japan) HPLC production-scale chromatography products in the U.S. last year. Custom production systems include pumping skids, sample loading devices, detection, fraction collection and software, says Seika president Ernest Sobkow. It also has dynamic axial compression systems (column and options) from (Kyoto, Japan) in diameters ranging from 5 to 60 cm and lengths of 70 and 100 cm. On the biochromatography side, Seika is positioning three different flow rate ranges for isocratic and gradient skids. The systems may be sold with production-scale glass columns from TechniKrom has shifted