Capillary Electrophoresis Equipment Specifications | GlobalSpec

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Help with Capillary Electrophoresis Equipment specifications:

Configuration


	Configuration:
	Your choices are...
	Capillary Only		The capillary where the actual separation takes place.
	System		The entire system including the capillary and detector.
	Detector		The detector without the system or capillaries.
	Search Logic:		All products with ANY of the selected attributes will be returned as matches. Leaving all boxes unchecked will not limit the search criteria for this question; products with all attribute options will be returned as matches.

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Capillary Physical Specifications


	Inner Diameter:		The inner diameter of the capillary.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Total Length:		The total length of the capillary.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Effective Length:		The length of the capillary where the actual separation takes place.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Material:
	Your choices are...
	Glass		Glass cannot be used with UV/Vis detection at wavelengths shorter than about 280 nm.
	Fused Silica		Fused silica can be used at shorter wavelengths, down to 190 nm. This allows detection of more compounds. Another advantage of fused silica capillaries is that they are flexible and not easily broken.
	Search Logic:		All products with ANY of the selected attributes will be returned as matches. Leaving all boxes unchecked will not limit the search criteria for this question; products with all attribute options will be returned as matches.

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System Performance Specifications


	Sample Volume:		Typical sample volumes are 1 to 50 nanoliters, but in some cases, volumes of up to about 1 microliter are injected.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Response Time:		Response times are how long a system or a functional unit takes to react to a given input. Times range from a few seconds to about an hour.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Accuracy:		Precision ranges of 1 to 2 relative standard deviations (%).
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Detection Limit:		Detection limit is the smallest amount of a substance that can be measured. It is usually defined as a signal that is at least three times the standard deviation of the background signal. The measurement of any signal is not perfect; there is always a certain amount of noise in the signal. Three standard deviations are a measure in terms of a range, which, in a large number of measurements, would include 99 % of all the measurements. So when it is stated that in order to be a detectable amount it must be a signal which measures higher than 3 standard deviations it means that that signal is higher than 99 % of the noise in the background. Detection limits are approximately 1 mg/L to 1 microgram/L.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

	Applied Voltage:		The voltage that is applied to the capillary to create the electroosmotic flow.
	Search Logic:		User may specify either, both, or neither of the "At Least" and "No More Than" values. Products returned as matches will meet all specified criteria.

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Detector Type


	Detector Type:
	Your choices are...
	UV/Vis Absorbance		UV/Vis absorbance is one of the most widely used detectors as samples can be detected while still in the capillary. A small section of the polyimide outer coating on the capillary is scrapped or burned off and that section is placed in the light beam of the detector. The clear section of the capillary serves as the cell window. These detectors are applicable for compounds that sufficiently absorb light.
	UV Diode		There are two major advantages of UV diode array detection. In the first, it allows for the best wavelength(s) to be selected for actual analysis. This is particularly important when no information is available on molar absorptivities at different wavelengths. The second major advantage is related to the problem of peak purity. Often, the peak shape in itself does not reveal that it actually corresponds to two (or even more) components. In such a case, absorbance rationing at several wavelengths is particularly helpful in deciding whether the peak represents a single compound or, is in fact, a composite peak.
	Fluorescence		Fluorescence is one of the most widely used detectors as samples can be detected while still in the capillary. A small section of the polyimide outer coating on the capillary is scrapped or burned off and that section is placed in the light beam of the detector. The clear section of the capillary serves as the cell window. These detectors are applicable for compounds that sufficiently absorb light.
	Laser Induced Fluorescence		Fluorescence induced by a laser. A small section of the polyimide outer coating on the capillary is scrapped or burned off and that section is placed in the light beam of the detector. The clear section of the capillary serves as the cell window. These detectors are applicable for compounds that sufficiently absorb light.
	Conductivity		The conductivity of the column effluent is continuously measured and the appearance of the analyte in the cell is indicated by a change in conductivity. This is usually a very low volume flow-through capillary equipped with two electrodes and variations in conductivity of the mobile phase due to the eluted sample components being continuously recorded. Response is linear with concentration over a wide range; quantification of the output signal is possible with suitable preliminary calibration. Best use is made of this detector in isocratic analysis since solvent gradients will cause a proportional shift in the baseline.
	Other		Mass spectrometry, amperometry, radiometric, or refractive index.
	Search Logic:		All products with ANY of the selected attributes will be returned as matches. Leaving all boxes unchecked will not limit the search criteria for this question; products with all attribute options will be returned as matches.

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Mode of Operation


	Mode of Operation:
	Your choices are...
	CZE - Capillary Zone Electrophoresis		Capillary zone electrophoresis (CZE), also known as free-solution CE (FSCE), is the simplest form of CE. The separation mechanism is based on differences in the charge-to-mass ratio of the analytes. Fundamental to CZE are homogeneity of the buffer solution and constant field strength throughout the length of the capillary. The separation relies principally on the pH-controlled dissociation of acidic groups on the solute or the protonation of basic functions on the solute.
	MEKC - Micellular Electrokinetic Chromatography		Micellular electrokinetic chromat (MECC OR MEKC) is a mode of electrokinetic chromatography in which surfactants are added to the buffer solution at concentrations that form micelles. The separation principle of MEKC is based on a differential partition between the micelle and the solvent. This principle can be employed with charged or neutral solutes and may involve stationary or mobile micelles. MEKC has great utility in separating mixtures that contain both ionic and neutral solutes.
	CGE - Capillary Gel Electrophoresis		Capillary gel electrophoresis (CGE) is the adaptation of traditional gel electrophoresis into the capillary using polymers in the solution as a molecular sieve. This allows solutes having similar charge-to-mass ratios to be resolved by size.
	CIEF - Capillary Isoelectric Focusing		Capillary isoelectric focusing (CIEF) allows amphoteric molecules, such as proteins, to be separated by electrophoresis in a pH gradient generated between the cathode and anode. A solute will migrate to a point where its net charge is zero. At this isoelectric point (the solute’s pI), migration stops and the sample is focused into a tight zone. In CIEF, once a solute has focused at its pI, the zone is mobilized past the detector by either pressure or chemical means.
	CITP - Capillary Isotachophoresis		Isoachoporesis (ITP) is a focusing technique based on the migration of the sample components between leading and terminating electrolytes. Solutes having mobilities intermediate to those of the leading and terminating electrolytes stack into sharp, focused zones. Although it is used as a mode of separation, transient ITP has been used primarily as a concentration technique prior to CZE separation.
	Other		CAPILLARY ELECTROCHROMATOGRAPHY (CEC), NON-AQUEOUS CAPILLARY ELECTROPHORESIS (NACE), ELECTROKINETIC CHROMATOGRAPHY (EKC)
	Search Logic:		All products with ANY of the selected attributes will be returned as matches. Leaving all boxes unchecked will not limit the search criteria for this question; products with all attribute options will be returned as matches.

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Features


	Can be Coupled with a Mass Spectrometer?		A mass spectrometer will provide an analysis based on the mass to charge ratio of the sample separated by the capillary.
	Search Logic:		"Required" and "Must Not Have" criteria limit returned matches as specified. Products with optional attributes will be returned for either choice.

	Pre-burned Window?		Many of the detectors can be positioned on the capillary. If the window is pre-burned, it is not necessary for the operator to create one.
	Search Logic:		"Required" and "Must Not Have" criteria limit returned matches as specified. Products with optional attributes will be returned for either choice.

	Automatic Temperature Compensation?		The system will compensate the readings for variations in temperature.
	Search Logic:		"Required" and "Must Not Have" criteria limit returned matches as specified. Products with optional attributes will be returned for either choice.

	Temperature Control?		In some applications, the sample temperature must be controlled to get a stable and correct reading. If there is a thermostat, the temperature can be controlled.
	Search Logic:		"Required" and "Must Not Have" criteria limit returned matches as specified. Products with optional attributes will be returned for either choice.

	Portable?		Not fixtured, can either be moved by carrying or by means of a cart.
	Search Logic:		"Required" and "Must Not Have" criteria limit returned matches as specified. Products with optional attributes will be returned for either choice.

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Environment


	Operating Temperature:		This is the full required range of ambient operating temperature.
	Search Logic:		User may specify either, both, or neither of the limits in a "From - To" range; when both are specified, matching products will cover entire range. Products returned as matches will meet all specified criteria.

	Operating Humidity:		This is the full required range of ambient operating humidity.
	Search Logic:		User may specify either, both, or neither of the limits in a "From - To" range; when both are specified, matching products will cover entire range. Products returned as matches will meet all specified criteria.

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