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Supplier: Bio-Rad Laboratories, Inc.
Description: DNA fragments ≥1,000 kb. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.
- Trigger: Base
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Supplier: Bio-Rad Laboratories, Inc.
Description: for DNA fragments ≥1,000 kb. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field
- Trigger: Acid, Base
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Supplier: Bio-Rad Laboratories, Inc.
Description: DNA fragments ≥1,000 kb. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.
- Trigger: Base
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Supplier: Bio-Rad Laboratories, Inc.
Description: DNA fragments ≤1,000 bp. This high-strength agarose forms gels that are easy to handle and that remain flexible even at high gel percentages, which minimizes the risk of cracking or breaking. PCR agarose, unlike GQT products with similar sieving properties that gel at higher
- Trigger: Base
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Tracing Clostridium Perfringens Strains from Beef Processing of Slaughter House by Pulsed-Field Gel Electrophoresis, and the Distribution and Toxinotype of Isolates in Shaanxi Province, China
The purpose of this study was to investigate the distribution and specify the transmission and cross-contamination of Clostridium perfringens (C. perfringens) in the beef slaughtering and butchering process.
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Molecular Typing in Bacterial Infections
Techniques such as pulse field gel electrophoresis (PFGE), restric- tion fragment length polymorphism (RFLP), PCR, and other specific assessments of DNA sequences such as chromosome comparison and ribosomal DNA compari- son can be used to characterize an organism by DNA “fingerprints.” .
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Molecular Detection of Human Bacterial Pathogens
Some other methods for bifidobacteria identification and typing used DNA profiling, such as plasmid analysis, RFLP, or PFGE ( pulsed field gel electrophoresis ) of total DNA.4,111,117 PFGE is often considered the best technique for strain-specific typification and has shown …
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Posters
Strains were subjected to analysis by Pulsed Field Gel Electrophoresis (BioRad), using restriction endonuclease SmaI, and by Optical Mapping (OpGen Inc., Gaithersburg, USA) to examine their genomic organization and to search for shared features.
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Pulsed-Field Gel Electrophoresis
1987) An electrophoretrc karyotype for Schzzosaccharomyce-s pombeby pulsed field gel electrophoresis .
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Rapid Detection of Infectious Agents
… frag- ment patterns for analysis [restriction-endonuclease analysis (REA)] by agarose gel electrophoresis, in some instances including hybridization with specific probes; (2) amplification ofchromosomal sequences by PCR; and (3) analysis ofchromo- somal macro-restriction fragment patterns by pulsed field gel electrophoresis (PFGE).
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Poster Sessions
Recovered isolates were characterised using phenotypic combination disc tests, multiplex PCR for the detection of blaTEM, blaSHV and blaCTX-M genes and pulsed field gel electrophoresis (PFGE).
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Bacterial Infections of Humans
Pulsed Field Gel Electrophoresis (PFGE).
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Advanced Techniques in Diagnostic Microbiology
Technical improvement to prevent DNA degradation of enteric pathogens in pulsed field gel elec- trophoresis .
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Molecular Bacteriology
Gardiner, K (199 1) Pulsed field gel electrophoresis Analytzcal Chem 63,658-665 58 Lefevre, J C , Faucon, G., Stcard, A. M., and Gasc, A M .
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Omics Technologies & Microbial Modeling for Foodborne Pathogens
The former includes phage typing and Multi-Locus Enzyme Electrophoresis (MLEE), and the latter includes Pulsed Field Gel Electrophoresis (PFGE) and Multi- Locus Sequence Typing (MLST).