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  • Environmental Microbiology: Fundamentals and Applications
    …598, 603, 605, 624, 625, 664, 665, 783–784, 799, 860, 861, 872, 880 Primers , 111, 150, 174 … 30, 32, 33, 79, 80, 82, 97, 98, 124, 128, 146, 161, 198, 209 , 210, 213, 223 .... , 506, 545, 599, 685, 741, 866, 867 Rift valley Eastern African, 386 Rio Tinto, 383–385, 741 RISA. .... 844, 853 Sexual pilus, 32, 312, 448, 634 Shannon alpha diversity index, 276 Shotgun , 833 Siderophore, 70…
  • Microbial Source Tracking: Methods, Applications, and Case Studies
    …M, et al (2005) An assessment of water quality and microbial risk in Rio Grande basin in .... 3.2: 209 –218. .... DGGE, 258–259 MST advantages, 274–275 critical issues, 275–276 qPCR-based methods, 277 rRNA primers , 277 MST toolkit .... NMDS, 271–272 OTU, 272 PhyloChip microarray, 259–261 PLFA, 256–258 shotgun cloning and sequencing, 256 TRFLP, 258, 268–270…
  • Cryptosporidium: parasite and disease
    Epidemiol Infect 115:207– 209 Xiao L, Ryan UM. .... of gastrointes- tinal protozoa in Didelphis albiventris (opossum) in the central region of Rio Grande do Sul .... This genome was sequenced to ~13-fold coverage using a shotgun Sanger sequencing approach (Table 6.1). .... which had been generated previously for this C. parvum isolate using ~200 oligonucleotide primers to screen haploid…
  • Mitogenome assembly from genomic multiplex libraries: comparison of strategies and novel mitogenomes for five species of frogs
    (2014) Integrated shotgun sequencing and bioinformatics pipeline allows ultra-fast mitogenome recovery and confirms substantial gene rearrangements .... (2013) Mitochondrial genome pri- mers for Lake Malawi cichlids. .... (1927) Neue Froschlurch aus Rio Grande do Sul, Brasilien. .... Gene, 238, 195– 209 .
  • Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide
    For the whole-genome shotgun libraries DNA fragments of 1.5–2.5, 1.5–3.5 and 2 .... All plas- mids were end-sequenced using the following primers : Forward and Reverse onABI sequencerABI3730XLorABI377 (Applied Biosystems .... Arch Microbiol 117: 209 –214. .... Glavina del Rio , T., Dalin, E., et al. (2008) Direct sequence submission Accession No.: NC_009943.
  • Molecular Detection of Human Bacterial Pathogens
    …et al., Immunochemical characterization of a haemagglutinating antigen of Arcobacter spp, FEMS Microbiol. Lett., 136, 209 , 1996. .... et al., Antigenic diversity among strains of Arcobacter spp isolated from pigs in Rio Grande do Sul,. .... expertise.117 Both techniques allow to use multiplex PCR by including vari- ous primer sets within a .... Shotgun sequencing .
  • Marker-Assisted Plant Breeding: Principles and Practices
    …Sci 8:554–560 Andolfatto P, Davison D, Erezyilmaz D et al (2011) Multiplexed shotgun genotyping for rapid .... Methods Mol Biol 1115:191– 209 Bachem CW, van der Hoeven RS, de Bruijn SM et al .... (1997) Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry. .... Mem Inst Oswaldo Cruz, Rio de Janeiro 97:23–26 Carollo V, Matthews DE, Lazo GR et al…
  • Molecular Detection of Human Fungal Pathogens
    Use of shotgun molecular methods and metage- nomic approaches will probably dig out a big number of … dye and amplicon-melting temperature anal- ysis for fungal detection using pan-fungal primers ITS1 forward (5′ .... 1996;34: 209 –214. .... largest epidemic of this mycosis due to zoonotic transmission has been described in Rio de Janeiro, Brazil…
  • Nucleic Acids Hybridization Modern Applications
    Theoretically, for many applications SH could become an alternative to shotgun genome sequencing (except for identification of .... If this divergence is high, oligonucleotide primers designed using the group consensus sequence may fail to prime .... Blanchette M, Green RE, Brenner SE, Rio DC. .... fragment length polymorphism 125, 126, 142, 146 annealing temperature 29, 32, 36, 44, 209 antisense transcription 265…
  • Microsatellites
    (2011) Mining of EST-SSR markers in clam Meretrix meretrix larvae from 454 shotgun transcrip- tome. .... S-H (2009) Optimizing System of SSR-PCR on Soyabean by Orthogonal design and SSR primer selection. .... Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. .... 209 Use of the MegaBACE .


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