Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale

Cloning, Gene Expression, and Protein Purification Experimental Procedures and Process Rationale

CHARLES HARDIN
JENNIFER EDWARDS
ANDREW RIELL
DAVID PRESUTTI
WILLIAM MILLER
DOMINIQUE ROBERTSON

North Carolina State University
New York Oxford
OXFORD UNIVERSITY PRESS
New York Oxford

2001

Athens Auckland Bangkok Bogot Buenos Aires Calcutta

Cape Town Chennai Dar es Salaam Delhi Florence Hong Kong Istanbul

Karachi Kuala Lumpur Madrid Melbourne Mexico City Mumbai

Nairobi Paris S o Paulo Shanghai Singapore Taipei Tokyo Toronto Warsaw

and associated companies in

Berlin Ibadan

2001 by Oxford University Press, Inc.

Published by Oxford University Press, Inc.
198 Madison Avenue, New York, New York 10016
http://www.oup-usa.org

Oxford is a registered trademark of Oxford University Press

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior permission of Oxford University Press.

Library of Congress Cataloging-in-Publication Data

Cloning, gene expression, and protein purification : experimental procedures and processrationale / Charles C. Hardin... [et al.].        p. cm.      Includes bibliographical references and index.

ISBN 0-19-513294-7 (alk. paper)

1. Molecular cloning Laboratory manuals. 2. Gene expression Laboratory manuals. 3. Proteins Purification Laboratory manuals. I. Hardin, Charles C.

QH442.2.C567 2001

572.8 dc21 00-045312

Printing (last digit): 9 8 7 6 5 4 3

Printed in the United States of America on acid-free paper

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