Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale

By et al.

List of Figures

Introductory Unit: Introduction to the Biochemical Laboratory

BCA redox-coupled reaction scheme:
Figure 1: Relative Frequency of occurrence of a measurement or its error.
Figure 2: A graphical representation of the t function for 10 results, where ( X) = 56.06, ? = 0.21, and ? = 0.10. Note that the risk that ? will be higher than 56.18 is half that of ? the same risk holds for ? being lower than 55.94. The probability is 90% that ? is within the confidence limits of 55.94 to 56.18.
Figure 1: Theoretical plot of ?pH versus [A ^-]/[HA] on two-cycle semilog.
Figure 1: Effect of common reagents on microwave BCA assay. Stock preparation of BSA was Triton-X 100 (TX-100), HEPES-NaOH, DTT, Tris-HCl (pH 8 and 11), glycine and ?-ME at the concentrations indicated in the text. Irradiation was carried out in a General Electric microwave oven. Data presented are mean + or - SD ( n = 7). Student's t-test was carried out to compare treated samples to control (BSA in water only), and statistically significant differences are indicated with asterisks.

Figure 1: EcoRI recognition sequence before and after enzyme action.
Figure 2: Subcloning a myo-3 restriction fragment obtained from plasmid B into the HindIII site created by cleavage at the site containing the ?-gal gene in plasmid A.
Figure 3: "Directionally cloning" a BamHI, HindIII myo-3 fragment in...

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