Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale
By et al.

List of Tables

Introductory Unit: Introduction to the Biochemical Laboratory

Table 1: Estimation of Standard Deviation from the Range: Formula: ? = k (range)
Table 2: Values of t for Calculating Confidence Limits
Table 3: The Q Test
Table 1: - Activity Coefficient of Some Ions in Aqueous Solution
Table 2: Physical Properties of Some Zwitterionic Buffers
Table 3: Dilutions to achieve proper pH

Unit 2: Construction of Recombinant Plasmids

Table 1: DNA Ligation Tips
Table 2: Reaction Parameters for Ligations.
Table 1: Kit Components

Unit 4: Transcription of Genomic DNA and Analysis of the Resulting mRNAS

Table 1: Volumes of PolyATtract System 1000 Reagents Required for Various Starting Amounts of Tissue (Small Scale)
Table 2: Volumes of PolyATtract System 1000 Reagents Required for Various Starting Amounts of Tissue (Large Scale)
Table 3: - Troubleshooting Chart

Unit 6: Analysis of DNA or RNA by Duplex Hybridization: DNA Isolation, Labeling, and Probing

Table 1: Modifications of the SCC Hybridization Protocol Proposed by Boehringer-Mannheim.
Table 2: Modifications of the Hybridization Protocol Described by Church and Gilbert 1 8
Table 1: Methods for Labeling Probes with Digoxigenin
Table 2: The amount of Synthesized DIG Labeled DNA Depends on the Amount of Template DNA in the Labeling Reaction and on the Length of the Incubation Time at 37 C
Table 3: Sensitivity Depends on Both the Concentration of Labeled DNA and Incubation Time
Table 4: Products Required
Table 5: Products Required
Table 6: Required Solutions
Table 7: The Amount of Synthesized Labeled DNA Depends on...
Copyright Oxford University Press, Inc. 2001 under license agreement with Books24x7
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