Chemical Analysis in the Laboratory: A Basic Guide

5: PRACTICAL EXERCISE

5 PRACTICAL EXERCISE

5.1 Measurement Uncertainty

In your notebook, prepare a worksheet like the one shown in Table 4.3. For this exercise you need:

  • Analytical balance reading to 0.0001 g (0.1 mg).

  • 25 cm 3 glass bulb pipette (Class B).

  • 100 cm 3 beaker.

  • Thermometer 0 to 100 C, reading to 0.1 C.

  • Distilled or deionised water (about 200 cm 3) in a conical flask.

  • Paper towel.

Table 4.3: Worksheet for exercise on measurement uncertainty in pipetting

Date

Balance checked and found OK

yes

no

Pipette checked for cleanliness and absence of chips

yes

no

Pipette filler function checked and found OK

yes

no

Thermometer function checked and found OK

yes

no

Results:

weight of nominal
25 cm 3 water by pipette

calculated volume
(using mean cm 3 g ?1)

  1. ________g

  2. ________g

  3. ________g

  4. ________g

  5. ________g

  6. ________g

  7. ________g

  8. ________g

  9. ________g

  10. ________g

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

________cm 3

Water temperature:
Start ________________ C
End ________________ C
Mean temp ________________ C
Mean cm 3 g ?1 ________________
(see footnote [ ] on page 49)

Total ________cm 3

Standard deviation ( s) ________________cm 3 (for an individual measurement)

Mean ________________cm 3

Uncertainty is expressed as standard deviation or relative standard deviation (%) (= standard deviation 100/mean)

The manufacturer's uncertainty for the 25 cm 3 pipette ( m) = cm 3

Combined...

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