Silica HPLC Columns

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  • Advantages of Silica Gels for HPLC Packing Applications
    For high-performance liquid chromatography (HPLC), in particular, accuracy is king. Column packing, separation and purification influence accuracy, making the quality of the silica gel used an incredibly important factor when processing difficult separations.
  • Separations Anxieties
    The majority of reversed-phase HPLC columns use a ?silyl ether? (Si-O-Si-) linkage to attach the bonded phase to the underlying silica. That Si-O-Si bond is subject to acid-catalyzed hydrolysis (the stoichiometry even works out: Si-O-Si + H2O --> SiOH + SiOH). The hydrophobic bonded phase shields
  • The Third Way for ICH Q7A: A Proposal for Updating Guidance for API Inspections
    . . . not to mention electricity is in play. Figure 4 shows a graphic representation of a column cross-section. In this case, silica lines the column. The electroosmotic flow is seen from anode to cathode. Figure 3. Schematic of capillary electrophoresis set-up. While not overly complex, it is not designed as "simple
  • Separations Anxieties
    Chromatography is a continuously evolving science, and not a month goes by that some column or media manufacturer doesn t tout the next big thing. In his 30 years in the business, Tom Jupille, founding consultant of , has seen enough to distinguish between the wheat and chaff. Jupille, who also

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