TABLE OF CONTENTS Fluorescence spectrometers are equivalent in their performance to single beam UV-visible spectrometers in that the spectra they produce are affected by solvent background and the optical characterises of the instrument. These effects can be overcome by using software built into the Perkin-Elmer LS-5B instrument or by using application software for use with the Perkin-Elmer models 3700 and 7700 computers.
The model LS-2B is a low-cost, easy to operate, filter fluorimeter that scans emission spectra over the wavelength range 390-700 nm (scanning) or 220-650 nm (individual interference filters).
In a filter fluorimeter the following are necessary:
A source of UV/visible energy (pulsed Xenon),
A method of isolating the excitation wavelength,
A means of discriminating between fluorescence and excitation energy, and
A sensitive detector and a digital display of the fluorescence intensity.
The model LS-2B has all these features arranged to optimise sensitivity for microsamples. It can also be connected to a highly sensitive 7 l liquid chromatographic detector for detecting the constituents in the column effluent. It has the capability of measuring fluorescence, time-resolved fluorescence, and bio- and chemiluminescence signals. A 40-portion autosampler is provided. An excitation filter kit containing six filters - 310, 340, 375, 400, 450 and 480 nm is available.
In many cases visible fluorescence techniques are less subject to interference by other polymer additives present in a polymer extract than are UV methods of analysis. Therefore, ...
