List of Figures

Chapter 1: Simultaneous Quantitation of Specific Proteins Using Immunoassay ICP-MS

Figure 1: (A, B) Calibration curves of the ICP-MS linked filter immunoassay using (A) 0 100ng/ml and (B) 0 5ug/ml of human IgG.
Figure 2: (A) Calibration curve of the ICP-MS-linked Protein A Sepharose immunoassay of human IgG. (B) Results of the quantification of the human IgG using Western blot and densitometry analysis. Each blot was exposed for 5- and 20-s intervals. Analyte concentrations were actually from 25 to 2500 ng/mL.).The results of two different exposures of the same blot are presented. Open circles, 5-s exposure; open squares, 20-s exposure.
Figure 3: Determination of IgG concentration in Human Serum. Standard IgG was prepared in triplicate in 0.25 mL of 1% BSA/PBS. At the same time, four serial dilutions of Immunology Level 1 Control and Level 2 Control were prepared intriplicate in 0.25 mL 1% BSA/PBS. A calibration curve was obtained using dilutions of the human IgG standard.
Figure 4: Eu/Ho signal ratio versus the AutoDELFIA signal for analysis of AFP, hCG and estriol. The correlation coefficients (r ²) between Eu/Ho signal ratios and the AutoDELFIA signal for the detection of AFP, hCG, and estriol were 0.9997, 0.9998, and 0.976, respectively. Open circles, duplicate calibration standards; open squares, low, medium and high standards; closed circles, patient samples.
Figure 5: Detection of multiple proteins using ICP-MS-linked maleylation immunoassay. (A-C) Three purified proteins (human IgG, FLAG-BAP, and GST-Smad2) were immobilized on maleic anhydride plates in concentration ranges of 1 50 ngper sample. The antigens were...

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Plasma Source Mass Spectrometry: Applications and Emerging Technologies

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Chapter 1: Simultaneous Quantitation of Specific Proteins Using Immunoassay ICP-MS

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