Physical Principles of Electron Microscopy: An Introduction to TEM, SEM, and AEM

Chapter 1: An Introduction to Microscopy

Overview

Microscopy involves the study of objects that are too small to be examined by the unaided eye. In the SI (metric) system of units, the sizes of these objects are expressed in terms of sub-multiples of the meter, such as the micrometer (1 ?m = 10 -6 m, also called a micron) and also the nanometer (1 nm = 10 -9 m). Older books use the Angstrom unit (1 = 10 -10 m), not an official SI unit but convenient for specifying the distance between atoms in a solid, which is generally in the range 2 3 .

To describe the wavelength of fast-moving electrons or their behavior inside an atom, we need even smaller units. Later in this book, we will make use of the picometer (1 pm = 10 -12 m).

The diameters of several small objects of scientific or general interest are listed in Table 1-1, together with their approximate dimensions.

Table 1-1: Approximate sizes of some common objects and the smallest magnification M* required to distinguish them, according to Eq. (1.5).

Object

Typical diameter D

M* = 75 ?m/ D

Grain of sand

1 mm = 1000 ?m

None

Human hair

150 ?m

None

Red blood cell

10 ?m

7.5

Bacterium

1 ?m

75

Virus

20 nm

4000

DNA molecule

2 nm

40,000

Uranium atom

0.2 nm = 200 pm

400,000

1.1 Limitations of the Human Eye

Our concepts...

UNLIMITED FREE
ACCESS
TO THE WORLD'S BEST IDEAS

SUBMIT
Already a GlobalSpec user? Log in.

This is embarrasing...

An error occurred while processing the form. Please try again in a few minutes.

Customize Your GlobalSpec Experience

Category: Optical Filters
Finish!
Privacy Policy

This is embarrasing...

An error occurred while processing the form. Please try again in a few minutes.