Biotechnology Procedures and Experiments Handbook

Chapter 6: Microbiology

INTRODUCTION

Laboratory Rules

For the safety and convenience of everyone working in the laboratory, it is important that the following laboratory rules be observed at all times:

  1. Place only those materials needed for the day s laboratory exercise on the benchtops.

  2. Since some of the microorganisms used in this class are pathogenic or potentially pathogenic (opportunistic), it is essential to always follow proper aseptic technique in handling and transferring all organisms.

  3. No eating, drinking, or any other hand-to-mouth activity while in the lab. If you need a short break, wash your hands with disinfectant soap and leave the room.

  4. Using a wax marker, properly label all inoculated culture tubes or petri plates with the name or initials of the microorganism you are growing, your initials or a group symbol, and any other pertinent information. Place all inoculated material only on your assigned incubator shelf. Culture tubes should be stored upright in plastic beakers, while petri plates should be stacked and incubated upside-down.

  5. Always clean the oil off of the oil immersion lens of the microscope with a piece of lens paper at the completion of each microscopy lab.

  6. Disinfect the benchtop with isopropyl alcohol before and after each lab period. Be sure your Bunsen burner is turned off before you spray any alcohol.

  7. Always wash your hands with disinfectant soap.

General Directions

  1. Familiarize yourself in advance with the procedure of the experiment to be performed.

  2. Disinfect the working table with isopropyl alcohol...

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