Biotechnology Procedures and Experiments Handbook

Appendix A: Units and Measures

SIZE

Cell biology deals with things that are relatively small. The units of measurement typically used are the micron at the light microscope level, and the nanometer at the electron microscope level. For molecular measurements, the norm is the Angstrom. These units are defined within the following table:

Measure

Symbol

Relative Length

Exponential Notation

Meter

M

1

10

Decimeter

dm

.1

10 ?1

Centimeter

cm

.01

10 ?2

Millimeter

mm

.001

10 3

Micrometer or micron

m

.000001

10 6

Nanometer

nm

.000000001

10 9

Angstrom

.0000000001

10 ?10

From this table it is apparent that:

  • 10 =1 nm

  • 1000 nm=1 mm

  • 10 mm=1 cm

Not apparent are that:

  • 1 inch=2.54 cm=25.4 mm=25400 m=25400000 nm

  • 1 inch=2.54 cm=2.54 10 1 mm=2.54 10 4 m=2.54 10 7 nm

  • 1 mm=0.04 inches.

BASIC UNITS USED IN BIOCHEMISTRY

Mole Units

  • 1 mole

  • 1 millimole (mmole)=10 ? 3 moles

  • 1 micromole ( mole)=10 ? 6 moles

  • 1 nanomole (nmole)=10 9 moles

  • 1 picomole (pmole)=10 ?12 moles

Gram Units

  • 1 gram (g)

  • 1 milligram (mg)=10 ? 3 g

  • 1 microgram ( g)=10 6 g

  • Concentration=weight/volume

  • 1 M (molar)=1 mole/liter or 1 mmole/mL

  • 1 mM=1 mmole/liter, etc.

Volume

Volumes are measured relative to a liter, with the most commonly used measurements, the milliliter, and the microliter. The following table provides the relative volumes:

Measure

Symbol

Relative Volume

Exponential Notation

Liter

L

1

10 7

Deciliter

dL

.1

10 ? 1

Milliliter

mL

.001

10 3

Microliter

L

.000001

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