Biotechnology Procedures and Experiments Handbook

Appendix B: Chemical Preparations

CHEMICALS OR REAGENTS REQUIRED FOR MOLECULAR BIOLOGY EXPERIMENTS

The following is a list of the solutions and chemicals required throughout the laboratory manual. It is organized alphabetically, and individual exercises list the materials needed for that exercise. For many solutions, directions are given for a molar solution and the user is left to dilute to the appropriate concentration for their needs. There are many vendors of the chemicals listed, and many of the solutions can also be purchased premixed.

Acetic acid (MW 60.05)

Glacial acetic acid is 99.6% (w/v) acetic acid, and is 17.4 M.

1 M

Add 57.5 mL of glacial acetic acid to 800 mL of water and then make up to 1 liter with water.

0.05 N

Add 2.87 mL of glacial acetic acid to 800 mL of water and then make up to 1 liter with water.

0.9 M

Add 54 mL of glacial acetic acid to 800 mL of water and then make up to 1 liter with water.

7% (w/v)

Add 70 mL of glacial acetic acid to 800 mL of water and then make up to 1 liter with water.

45% (w/v)

Add 450 mL of glacial acetic acid to 500 mL of water and then make up to 1 liter with water.

Acetic acid/butanol/water (15:60:25)

Combine 150 mL of glacial acetic acid, 600 mL of butanol, and 250 mL of water.

Aceto-orcein

Add 2.0 grams of orcein to 45 mL of glacial acetic acid. Bring to a boil and continue to heat...

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