Materials

• Microscope with dark-field stop

• Suspension of Amoeba proteus

• Transfer pipette

• Slides and coverslips

Procedure

1. Make a simple wet mount of the amoeba by placing a drop of the culture on a slide and placing a cover slip on the slide.

2. Observe and measure the amoeba using normal bright field optics.

3. Place a dark field stop in the filter holder below the microscope s substage condenser and continue to monitor the movements of the amoeba.

4. Note the differences in observable structure possible with dark-field microscopy when compared to bright field. Draw and label the amoeba viewed in both ways.