TABLE OF CONTENTS | Ethyl alcohol (95%) | 97.0 mL |
| HCl | 3.0 mL |
| Methylene blue | .3 gm |
| Distilled water | 100.0 mL |
| Crystal violet (1%) | |
| Crystal violet (85% dye content) | 1.0 gm |
| Distilled water | 100.0 mL |
| Coppersulfate (CuSO 4) | 20.0 gm |
| Distilled water | 80.0 mL |
Lactophenol-cotton blue solution
| Lactic acid | 20.0 mL |
| Phenol | 20.0 gm |
| Glycerol | 40.0 mL |
| Distilled water | 20.0 mL |
| Aniline Blue | 0.05 gm |
| Note | Heat gently to dissolve in hot water. Then add aniline blue dye. |
| Grams iodine | 10.0 mL |
| Distilled water | 30.0 mL |
Crystal violet
| Crystal violet (90% dye content) | 2.0 gm |
| Ethyl alcohol (95%) | 20.0 mL |
| Ammonium oxalate | 0.8 gm |
| Distilled water | 80.0 mL |
| Mix solutions A and B | |
| Gram s iodine | |
| Iodine | 1.0 gm |
| Potassium iodide | 2.0 gm |
| Distilled water | 300 mL |
| Ethyl alcohol (100%) | 95.0 mL |
| Distilled water | 5.0 mL |
| Safranin | 0.25 mL |
| Ethyl alcohol (95%) | 10.0 mL |
| Distilled water | 100 mL |
| Nigrosin, Water-soluble | 10.0 gm |
| Distilled water | 100 mL |
| Immerse in boiling water bath for 30 minutes. | |
| Formalin | 0.5 mL |
| Filter twice through double-filter paper. |
| Malachite green | 5.0 gm |
| Distilled water | 100 mL |
| Safranin | 0.25 mL |
| Ethyl alcohol (95%) | 10.0 mL |
| Distilled water | 100 mL |
| Carbol fuchsin | |
| Solution A | |
| Basic fuchsin | 0.3 gm |
| Ethyl alcohol (95%) | 10.0 mL |
| Solution B | |
| Phenol | 5.0 mL |
| Distilled water |
