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  • Advantages of Silica Gels for HPLC Packing Applications
    For high-performance liquid chromatography (HPLC), in particular, accuracy is king. Column packing, separation and purification influence accuracy, making the quality of the silica gel used an incredibly important factor when processing difficult separations.
  • Separations Anxieties
    The majority of reversed-phase HPLC columns use a ?silyl ether? (Si-O-Si-) linkage to attach the bonded phase to the underlying silica. That Si-O-Si bond is subject to acid-catalyzed hydrolysis (the stoichiometry even works out: Si-O-Si + H2O --> SiOH + SiOH). The hydrophobic bonded phase shields
  • USP Tests to be Updated
    of the things I've seen over the years are incredible. I've actually seen people adulterate with materials they know will stick to an HPLC column and not come off, and so then when it comes to us - "Oh, it 's a single peak. It 's pure. " Well, I think the approach we 're taking is to try to write
  • The Third Way for ICH Q7A: A Proposal for Updating Guidance for API Inspections
    . . . not to mention electricity is in play. Figure 4 shows a graphic representation of a column cross-section. In this case, silica lines the column. The electroosmotic flow is seen from anode to cathode. Figure 3. Schematic of capillary electrophoresis set-up. While not overly complex, it is not designed as "simple
  • Separations Anxieties
    Chromatography is a continuously evolving science, and not a month goes by that some column or media manufacturer doesn t tout the next big thing. In his 30 years in the business, Tom Jupille, founding consultant of , has seen enough to distinguish between the wheat and chaff. Jupille, who also
  • Therapeutic Dose: Chromatography Dreams
    online, calls Primesep the biggest breakthrough in HPLC in 25 years! The claim, by one of the column s enthusiasts, might be exaggerated but has generated a fairly spirited discussion. Several chromatographers wrote in to say that Primesep, indeed, was a unique tool. The column isn t manufactured
  • Chromatography Roundup
    chromatography. This allows just one to two liters of Sartobind to clear contaminants and viruses from a 15,000-liter MAb production run. A 1- or 2-liter membrane is approximately 200 times smaller than a column for the same unit operation, and the membrane can process five to 10 times faster, Mora says
  • SMB Takes On Paclitaxel Purification
    studies led to modifications in the amount of stationary phase packed in the SMB columns, along with solvent composition, loading concentration, feed and take-off rates. The SMB process is controlled through real-time, near-line, fast HPLC analysis of the extract and raffinate evaporate streams

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