G.BARLOCCHI, U.MASTROMATTEO, S.SASSOLINI, M.SCURATI, F.VILLA
STMicroelectronics, via Tolomeo, 1, 20010 Cornaredo, Italy
E-mail: ubaldo.mastromatteo@st.com
Real Time PCR [1], where the amount of the product is measured during outgoing amplification, is the most sensitive method to determine the quantity of a specific DNA in a sample.
The Real-Time PCR advantages come from data collection during the exponential growth phase, determining an increase in reporter fluorescent signal directly proportional to the number of product generated. Further more there is an increased dynamic range of detection and no-post PCR processing.
DNA polymerase uses single-stranded DNA as a template for the synthesis of a complementary new strand.
The starting material for a PCR is DNA that contains the sequence to be amplified and it is not necessary to isolate the sequence to be amplified because it is defined by the primers used in the reaction.
The amount of DNA needed for a PCR is very small. The total mixture for a Lab...
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