Analytical Analysis High Performance Liquid Chromatographs (HPLC)
Description
High Performance Liquid Chromatographs (HPLC) are sophisticated instruments used to separate, identify, and quantify components in a mixture. They achieve this by passing a liquid sample through a column filled with a solid adsorbent material, where different components of the sample move at different rates, allowing for their separation.
Working Principle
HPLC operates on the principle of liquid chromatography, where a sample is dissolved in a liquid (the mobile phase) and passed through a column containing a solid adsorbent material (the stationary phase). The different components of the sample interact with the stationary phase to varying degrees, causing them to elute at different times. This separation is detected and recorded, often using detectors that measure changes in light intensity or other properties as the components exit the column. HPLC is particularly useful due to its high resolution, speed, and ability to handle complex mixtures.
Applications
HPLC is widely used in the food industry for qualitative and quantitative analysis of food content, including the detection of vitamin C, food additives, contaminants, and spoilage . It is also employed in the pharmaceutical industry for drug development and quality control, as well as in environmental analysis for detecting pollutants.
Advantages over other High Performance Liquid Chromatographs (HPLC)
HPLC systems with advanced detectors, such as those combining ultra-high pressure liquid chromatography (UHPLC) with mass spectroscopy (MS) and high-resolution mass spectroscopy (HRMS), offer extreme accuracy and valuable information depth . These systems can provide superior separation and quantification capabilities compared to traditional HPLC setups.
Limitations
One limitation of HPLC is the requirement for high-pressure pumps to maintain the flow rate necessary to counteract the back pressure generated by the packed column . Additionally, the complexity and cost of the equipment can be prohibitive for some applications.
Considerations
When considering HPLC systems, factors such as initial costs, operating expenses, and maintenance should be evaluated. HPLC systems can be expensive to purchase and operate, with costs associated with solvents, columns, and regular maintenance. Durability and accuracy are critical, as these systems must consistently deliver precise results. Replacement and maintenance costs can also be significant, particularly for high-performance detectors and pumps.
from Waters Corporation
The Next Era in Polymer Separation. Highest-resolution, size-based separations for chromatographic polymer characterization. Improve data consistency and quality by enabling fast, daily calibration. Automate method development with advanced system technologies. Get more information about your... [See More]
- Analysis Method: Analytical
- Detector Type: Mass Spectroscopy
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Hitachi High-Tech America
Outstanding Performance. Two performance capabilities supporting data reliability: excellent reproducibility made possible by the pump and autosampler and excellent stability of the column oven and detector. Easy-to-use. Along with simple operation and ease of use, Chromaster offers ease of... [See More]
- Analysis Method: Analytical
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Mass Spectroscopy
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
The high performance 2D micro-HPLC system can perform separation of complex peptide mixtures with a level of sensitivity and resolution that meets or exceeds present-day requirements.This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH (National Institutes... [See More]
- Analysis Method: Analytical
- Detector Type: Ultraviolet/Visible Light Detector (optional feature); Refractive Index (optional feature)
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
HPLC and UHPLC method compatibility at the flip of a switch. The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support... [See More]
- Analysis Method: Analytical
- Remote Interface: Computer Interface
- Instrument Type: Fixtured
- General Features: Programmable
from Hitachi High-Tech America
Developed with the user in mind, the AminoSAAYA offers easy and comfortable operability while maintaining the reliability and stability of Hitachi ’s legacy amino acid analyzers. Ergonomic Design. Designed from the perspective of the user, the AminoSAAYA ’s daily workflow, movements, and... [See More]
- Analysis Method: Analytical
- Detector Type: Spectrophotometer: Aberration-corrected
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump
from Waters Corporation
The ease of reversed phase meets the power of normal phase. With the ACQUITY UPC2 System, the orthogonal capability of normal-phase LC has been elevated to a mainstream technique: Convergence Chromatography. The chromatographic principles and selectivity of normal-phase LC. The ease-of-use and... [See More]
- Analysis Method: Analytical
- Remote Interface: Computer Interface
- Instrument Type: Fixtured
from Shimadzu Scientific Instruments, Inc.
Shimadzu's Discovery VP Analytical System enables characterization of microgram to milligram quantities of lead candidates generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, or Organic Synthesis efforts. Up to ten columns and four solvent pairs can be configured, making... [See More]
- Analysis Method: Analytical
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering; Mass Spectroscopy; PDA
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
Ready for your biomolecular analysis. For laboratories that know biomolecules sometimes need to work harder to move through a chromatographic instrument, the biocompatible ACQUITY UPLC ® H-Class Bio System is ready. Engineered with a bio-inert flow path made of non-stainless-steel materials, the... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
An economical option for gradient analysis is a quaternary HPLC system with a single pump and valve to mix up to four solvents. The Nexera quaternary system operates at analytical to semi-prep flows (max. 10 mL/min) and throughout the HPLC and UHPLC pressure range, up to 19,000 psi, with no... [See More]
- Analysis Method: Analytical
- Detector Type: Ultraviolet/Visible Light Detector; Fluorescence
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
Highest resolution of any quaternary LC system. Improve your laboratory ’s efficiency by scaling your methods to UPLC Technology. Use UPLC ’s unmatched resolution to get better characterization of your complex samples. Take advantage of tools that simplify and streamline your method... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
Realization of Advanced Laboratory. From setting vial to starting analysis, all the procedures are performed via the equipment. The operations in the laboratory are minimized and free of errors. The system status can be supervised even from outside the laboratory. More efficient use of laboratory... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump
from Waters Corporation
The most powerful binary UPLC system, with the best dispersion, on the market. The ideal binary-based system for your demanding applications, with reduced cycle times for high-throughput applications. Low dispersion design allows complex separations to be accelerated without compromising... [See More]
- Analysis Method: Analytical
- Detector Type: Mass Spectroscopy (optional feature)
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
Proteome analysis, an exhaustive analytical technique for analyzing proteins, is primarily conducted using ESI-LCMS or MALDI-IT-TOF MS. Many proteins and peptides are extremely minute in quantity, requiring the enhanced sensitivity provided by mass spectrometers. Therefore, there is great demand for... [See More]
- Analysis Method: Analytical
- Detector Type: LCMS-IT-TOF
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
LET YOUR LC INLET DO THE WORK FOR YOUR NANO- TO MICROSCALE SEPARATIONS. System of choice for sample-limited applications in proteomics and biomarker discovery. Higher sensitivity for any MS. Maximum amount of data from any sample. Less sample preparation volume and cost. Reduced solvent consumption. [See More]
- Analysis Method: Analytical
- Detector Type: Mass Spectroscopy (optional feature)
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
HPLC systems are currently used in a wide variety of fields. Higher reliability in analysis data and higher efficiency in total analysis workflow are required for faster development of new drugs, for better food safety, and for meeting higher standards in environmental regulations. Many analysis... [See More]
- Analysis Method: Analytical; Preparative
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Light Scattering; Optional PDA
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
Expand the dynamic range of your analysis with 2D separations.The ACQUITY UPLC ® M-Class System with 2D Technology expands the use of sub-2-micron particles to achieve high peak capacity separations. This innovative system effectively uses two-dimensional (2D) UPLC for better chromatographic... [See More]
- Analysis Method: Analytical
- Detector Type: Mass Spectroscopy (optional feature)
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
Nexera UC improves your analytical workflow by utilizing a completely new separation technology, Unified Chromatography, which unites sample separation, analysis with various separation modes, and high-sensitivity detection. [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump
from Waters Corporation
Monitor protein conformation with HDX technology. The ACQUITY UPLC ® M-Class System with HDX Technology leverages UPLC ® separations and high-resolution MS to answer important questions about changes in protein conformation including: Drug candidate binding to a protein. Biopharmaceutical... [See More]
- Analysis Method: Analytical
- Detector Type: Mass Spectroscopy
- Instrument Type: Fixtured
- Maximum Pump Pressure: 15000
from Shimadzu Scientific Instruments, Inc.
Method scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump
from Waters Corporation
UPLC transforms the expectations of laboratories worldwide. UPLC Technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is highly robust, dependable, and reproducible. Work at higher efficiencies with a much wider range of linear... [See More]
- Analysis Method: Analytical
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Light Scattering; Mass Spectroscopy
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
Maximizing the Potential of UHPLC/HPLC Analysis. Meet the Nexera X2, the most advanced UHPLC available today. The flexible system design achieves a true fusion between UHPLC and HPLC technologies, enabling the Nexera X2 to be used for a much broader range of applications. This completely new UHPLC... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump
from Waters Corporation
2D LC technology for UPLC/UHPLC separations. ACQUITY UPLC Systems with 2D LC Technology offer the lowest dispersion system for maximum peak capacity with the most reliable, highest performing solvent managers and valve compartments on the market to ensure accuracy and precision of the entire assay. [See More]
- Analysis Method: Analytical
- Remote Interface: Computer Interface
- Instrument Type: Fixtured
from Shimadzu Scientific Instruments, Inc.
The Nexera SR UHPLC system is built around the SPD-M30A photodiode array detector, which has several new features for unmatched performance in sensitivity and stability, linearity, spectral resolution, and peak deconvolution. This high-end system is designed to produce the best possible results for... [See More]
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
- Instrument Type: Fixtured
- Flow Control: Gradient Pump