Fixtured or Permanent High Performance Liquid Chromatographs (HPLC)
Description
Fixtured or Permanent High Performance Liquid Chromatographs (HPLC) are sophisticated analytical instruments designed to separate, identify, and quantify components in a liquid sample. These devices are equipped with features such as self-calibration, self-test diagnostics, data storage, and programmability, making them highly efficient for continuous and reliable operation in various settings.
Working Principle
The working principle of HPLC involves passing a liquid sample through a column packed with a stationary phase. A mobile phase, which is a solvent, is pumped through the column, carrying the sample with it. As the sample moves through the column, different components interact with the stationary phase to varying degrees, causing them to separate. Detectors then measure changes in the composition of the eluate, generating a chromatogram that provides insights into the sample's composition. This process is highly useful for its ability to provide high-resolution separation and quantification of complex mixtures.
Applications
HPLC is widely used in various fields for specific applications. For instance, in pharmaceuticals, it is employed for the quality control of drugs by analyzing the purity and concentration of active ingredients. In environmental science, HPLC is used to detect pollutants in water samples. Additionally, in the food industry, it helps in identifying additives and contaminants in food products.
Advantages over other High Performance Liquid Chromatographs (HPLC)
Fixtured or Permanent HPLCs offer several advantages over other types of HPLCs. They provide high reproducibility and stable conditions, which are crucial for consistent results. These systems are also compatible with a wide variety of consumables and detectors, enhancing their flexibility and adaptability to different analytical needs. Furthermore, they do not require a fume hood if the fraction collector is closed, making them more convenient for laboratory environments.
Limitations
Despite their advantages, fixtured or permanent HPLCs have certain limitations. The initial instrument and maintenance costs can be high, which may be a barrier for some laboratories. Additionally, the cost of consumables can add to the overall expense of operating these systems.
Considerations
When considering the implementation of a fixtured or permanent HPLC system, several factors should be taken into account. The initial costs can be significant, so budgeting for both the purchase and installation is essential. Operating expenses, including consumables and maintenance, should also be considered. These systems are generally durable and provide high accuracy, but regular maintenance is necessary to ensure optimal performance. Replacement parts and service contracts can also contribute to the long-term costs of ownership.
from Hitachi High Technologies America, Inc.
The Chromaster HPLC system from Hitachi has established new limits in Performance, Ease of Use, and Robustness. Innovative low-pressure gradient mode, "High Frequent Mode," and Hitachi's proprietary real-time feedback control system greatly suppress liquid pulsation resulting in unparalleled... [See More]
- Instrument Type: Fixtured
from Shimadzu Scientific Instruments, Inc.
The high performance 2D micro-HPLC system can perform separation of complex peptide mixtures with a level of sensitivity and resolution that meets or exceeds present-day requirements.This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH (National Institutes... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector (optional feature); Refractive Index (optional feature)
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Hitachi High-Tech America
Outstanding Performance. Two performance capabilities supporting data reliability: excellent reproducibility made possible by the pump and autosampler and excellent stability of the column oven and detector. Easy-to-use. Along with simple operation and ease of use, Chromaster offers ease of... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Mass Spectroscopy
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Waters Corporation
The Next Era in Polymer Separation. Highest-resolution, size-based separations for chromatographic polymer characterization. Improve data consistency and quality by enabling fast, daily calibration. Automate method development with advanced system technologies. Get more information about your... [See More]
- Instrument Type: Fixtured
- Detector Type: Mass Spectroscopy
- Analysis Method: Analytical
- Remote Interface: Computer Interface
from Hitachi High Technologies America, Inc.
The Hitachi HPLC Primaide is designed for long-term, stable operation, and features both high reliability and great durability. It offers tremendous capability for everyday analysis.Based on over 50 years of experience in the development and production of HPLC systems, Hitachi proudly introduces the... [See More]
- Instrument Type: Fixtured
from Shimadzu Scientific Instruments, Inc.
The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Hitachi High-Tech America
Developed with the user in mind, the AminoSAAYA offers easy and comfortable operability while maintaining the reliability and stability of Hitachi ’s legacy amino acid analyzers. Ergonomic Design. Designed from the perspective of the user, the AminoSAAYA ’s daily workflow, movements, and... [See More]
- Instrument Type: Fixtured
- Detector Type: Spectrophotometer: Aberration-corrected
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Waters Corporation
HPLC and UHPLC method compatibility at the flip of a switch. The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support... [See More]
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
- Analysis Method: Analytical
- General Features: Programmable
from Hitachi High Technologies America, Inc.
ChromasterUltra Rs, an ultra high performance liquid chromatograph, provides the ultimate in high performance, high resolution, and high sensitivity analysis that are becoming requirements for research and development in pharmaceutical and chemical fields. World-class analysis when combined with the... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Diode array detector (DAD) system
from Shimadzu Scientific Instruments, Inc.
A forced-air circulation-type column oven, the CTO-20A can regulate the temperature from 10ºC above room temperature to 85ºC. Rapid temperature equilibration allows complex temperature programs incorporating linear and/or step-wise increases and decreases in temperature. [See More]
- Instrument Type: Fixtured
- Display: Digital
- User Interface: Digital
- General Features: Programmable; Built-in or Self Calibration
from Waters Corporation
The ease of reversed phase meets the power of normal phase. With the ACQUITY UPC2 System, the orthogonal capability of normal-phase LC has been elevated to a mainstream technique: Convergence Chromatography. The chromatographic principles and selectivity of normal-phase LC. The ease-of-use and... [See More]
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
- Analysis Method: Analytical
from Shimadzu Scientific Instruments, Inc.
Shimadzu's Discovery VP Analytical System enables characterization of microgram to milligram quantities of lead candidates generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, or Organic Synthesis efforts. Up to ten columns and four solvent pairs can be configured, making... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering; Mass Spectroscopy; PDA
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Waters Corporation
Ready for your biomolecular analysis. For laboratories that know biomolecules sometimes need to work harder to move through a chromatographic instrument, the biocompatible ACQUITY UPLC ® H-Class Bio System is ready. Engineered with a bio-inert flow path made of non-stainless-steel materials, the... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
The Discovery VP preparative system enables purification and collection of milligram to gram quantities of compounds generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, and Organic Synthesis efforts. Multiple fraction collectors increase throughput and facilitate high... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering
- Analysis Method: Preparative
- Injection Method: Auto-sampler Injection
from Waters Corporation
Highest resolution of any quaternary LC system. Improve your laboratory ’s efficiency by scaling your methods to UPLC Technology. Use UPLC ’s unmatched resolution to get better characterization of your complex samples. Take advantage of tools that simplify and streamline your method... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
An economical option for gradient analysis is a quaternary HPLC system with a single pump and valve to mix up to four solvents. The Nexera quaternary system operates at analytical to semi-prep flows (max. 10 mL/min) and throughout the HPLC and UHPLC pressure range, up to 19,000 psi, with no... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Fluorescence
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Waters Corporation
The most powerful binary UPLC system, with the best dispersion, on the market. The ideal binary-based system for your demanding applications, with reduced cycle times for high-throughput applications. Low dispersion design allows complex separations to be accelerated without compromising... [See More]
- Instrument Type: Fixtured
- Detector Type: Mass Spectroscopy (optional feature)
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
Realization of Advanced Laboratory. From setting vial to starting analysis, all the procedures are performed via the equipment. The operations in the laboratory are minimized and free of errors. The system status can be supervised even from outside the laboratory. More efficient use of laboratory... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Waters Corporation
LET YOUR LC INLET DO THE WORK FOR YOUR NANO- TO MICROSCALE SEPARATIONS. System of choice for sample-limited applications in proteomics and biomarker discovery. Higher sensitivity for any MS. Maximum amount of data from any sample. Less sample preparation volume and cost. Reduced solvent consumption. [See More]
- Instrument Type: Fixtured
- Detector Type: Mass Spectroscopy (optional feature)
- Analysis Method: Analytical
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
Proteome analysis, an exhaustive analytical technique for analyzing proteins, is primarily conducted using ESI-LCMS or MALDI-IT-TOF MS. Many proteins and peptides are extremely minute in quantity, requiring the enhanced sensitivity provided by mass spectrometers. Therefore, there is great demand for... [See More]
- Instrument Type: Fixtured
- Detector Type: LCMS-IT-TOF
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Waters Corporation
Expand the dynamic range of your analysis with 2D separations.The ACQUITY UPLC ® M-Class System with 2D Technology expands the use of sub-2-micron particles to achieve high peak capacity separations. This innovative system effectively uses two-dimensional (2D) UPLC for better chromatographic... [See More]
- Instrument Type: Fixtured
- Detector Type: Mass Spectroscopy (optional feature)
- Analysis Method: Analytical
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
HPLC systems are currently used in a wide variety of fields. Higher reliability in analysis data and higher efficiency in total analysis workflow are required for faster development of new drugs, for better food safety, and for meeting higher standards in environmental regulations. Many analysis... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Light Scattering; Optional PDA
- Analysis Method: Analytical; Preparative
- Injection Method: Auto-sampler Injection
from Waters Corporation
Monitor protein conformation with HDX technology. The ACQUITY UPLC ® M-Class System with HDX Technology leverages UPLC ® separations and high-resolution MS to answer important questions about changes in protein conformation including: Drug candidate binding to a protein. Biopharmaceutical... [See More]
- Instrument Type: Fixtured
- Detector Type: Mass Spectroscopy
- Analysis Method: Analytical
- Maximum Pump Pressure: 15000
from Shimadzu Scientific Instruments, Inc.
Nexera UC improves your analytical workflow by utilizing a completely new separation technology, Unified Chromatography, which unites sample separation, analysis with various separation modes, and high-sensitivity detection. [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Waters Corporation
UPLC transforms the expectations of laboratories worldwide. UPLC Technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is highly robust, dependable, and reproducible. Work at higher efficiencies with a much wider range of linear... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Light Scattering; Mass Spectroscopy
- Analysis Method: Analytical
- Remote Interface: Computer Interface