Ultraviolet / Visible Light Detector High Performance Liquid Chromatographs (HPLC)
Description
Ultraviolet/Visible Light Detector High Performance Liquid Chromatographs (HPLC) are analytical instruments used to separate, identify, and quantify components in a mixture. They utilize ultraviolet (UV) and visible light detectors to measure the absorption of light by the sample as it passes through the HPLC system, generating a chromatogram that represents the concentration of different components.
Working Principle
HPLC systems with UV/Visible light detectors operate by passing a beam of light through the column effluent as it flows through a low-volume flow cell. The detectors measure the ability of the sample to absorb light at specific wavelengths. Variations in light intensity are recorded, and a chromatogram is generated, which helps in identifying and quantifying the components of the sample. These detectors are useful because they provide a non-destructive means of analysis, allowing for the detection of compounds based on their light absorption properties, which is crucial for understanding the composition of complex mixtures.
Applications
HPLC systems with UV/Visible light detectors are widely used in various fields. In the food industry, they are employed for qualitative and quantitative analysis of food content, detecting components such as vitamin C, food additives, contaminants, and spoilage . These systems are also crucial in pharmaceutical research for analyzing drug compounds and in environmental monitoring for detecting pollutants.
Advantages over other High Performance Liquid Chromatographs (HPLC)
One of the main advantages of using UV/Visible light detectors in HPLC systems is their ability to provide accurate and detailed information about the sample composition. This is particularly beneficial when combined with techniques like mass spectroscopy, which enhances the depth and value of the information obtained . Additionally, these detectors offer a broad dynamic range, allowing for the quantification of unknown samples using a multi-point calibration curve .
Limitations
A limitation of UV/Visible light detectors in HPLC systems is their reliance on the sample's ability to absorb light at specific wavelengths. This means that compounds that do not have chromophores or do not absorb UV/Visible light effectively may not be detected or quantified accurately. Furthermore, the initial cost and complexity of the system can be a barrier for some users.
Considerations
When considering the use of UV/Visible light detector HPLC systems, it is important to evaluate factors such as initial costs, operating expenses, and maintenance requirements. These systems can be expensive to purchase and maintain, requiring regular calibration and potential replacement of parts to ensure accuracy and reliability. Additionally, the durability and accuracy of the system should be assessed, as these factors will impact the long-term usability and effectiveness of the instrument.
from Hitachi High Technologies America, Inc.
ChromasterUltra Rs, an ultra high performance liquid chromatograph, provides the ultimate in high performance, high resolution, and high sensitivity analysis that are becoming requirements for research and development in pharmaceutical and chemical fields. World-class analysis when combined with the... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Diode array detector (DAD) system
- Instrument Type: Fixtured
from Shimadzu Scientific Instruments, Inc.
Shimadzu's Discovery VP Analytical System enables characterization of microgram to milligram quantities of lead candidates generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, or Organic Synthesis efforts. Up to ten columns and four solvent pairs can be configured, making... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering; Mass Spectroscopy; PDA
- Analysis Method: Analytical
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Hitachi High-Tech America
Outstanding Performance. Two performance capabilities supporting data reliability: excellent reproducibility made possible by the pump and autosampler and excellent stability of the column oven and detector. Easy-to-use. Along with simple operation and ease of use, Chromaster offers ease of... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Mass Spectroscopy
- Analysis Method: Analytical
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
UPLC transforms the expectations of laboratories worldwide. UPLC Technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is highly robust, dependable, and reproducible. Work at higher efficiencies with a much wider range of linear... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Light Scattering; Mass Spectroscopy
- Analysis Method: Analytical
- Instrument Type: Fixtured
- Remote Interface: Computer Interface
from Shimadzu Scientific Instruments, Inc.
The Discovery VP preparative system enables purification and collection of milligram to gram quantities of compounds generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, and Organic Synthesis efforts. Multiple fraction collectors increase throughput and facilitate high... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering
- Analysis Method: Preparative
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
from Waters Corporation
Defined by dependability - today, and in the future. Time-tested, flexible, and reliable workhorse for your HPLC separations. Integrated solvent and sample management with consistent system-to-system performance and high reproducibility. A history of continued system improvements ensures the... [See More]
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Electrochemical; Light Scattering; Mass Spectroscopy
- Analysis Method: Analytical
- Instrument Type: Fixtured
- User Interface: Digital