Built in Chromatograph High Performance Liquid Chromatographs (HPLC)
Description
Built-in Chromatograph High Performance Liquid Chromatographs (HPLC) are sophisticated analytical instruments designed to separate, identify, and quantify components in a mixture. These devices are equipped with integrated chromatographs and application software, allowing for efficient data processing and analysis. They often feature self-calibration, self-test diagnostics, data storage, and programmability, making them versatile tools in various analytical settings .
Working Principle
HPLC operates by passing a liquid sample through a column packed with a stationary phase. The sample components interact with the stationary phase and are separated based on their different affinities. A detector then measures the separated components as they elute from the column, generating a chromatogram. This process is highly useful because it provides precise and accurate measurements of complex mixtures, making it invaluable in fields requiring detailed chemical analysis .
Applications
HPLC is widely used in food safety analysis to detect components such as vitamin C, food additives, contaminants, and spoilage. The combination of HPLC with mass spectroscopy (MS) and high-resolution mass spectroscopy (HRMS) enhances its accuracy and the depth of information it can provide, making it a critical tool in ensuring food safety .
Advantages over other High Performance Liquid Chromatographs (HPLC)
Built-in chromatograph HPLCs offer several advantages, including the integration of application software, which streamlines the analysis process. They also provide features like self-calibration and self-test diagnostics, which enhance reliability and reduce the need for manual intervention. These devices can operate under extreme temperatures and offer various output options, such as analog voltage and digital interfaces, making them adaptable to different analytical requirements .
Limitations
One limitation of HPLC systems is the requirement for a high-pressure pump to maintain the flow rate necessary to counter the back pressure generated by the stationary phase. Additionally, the system's complexity can lead to higher maintenance needs and potential operational challenges if not properly managed .
Considerations
When considering the purchase and operation of an HPLC system, several factors should be taken into account. Initial costs can be significant due to the complexity and precision of the equipment. Operating expenses include the cost of solvents, maintenance, and potential repairs. Durability and accuracy are generally high, but regular maintenance is essential to ensure consistent performance. Replacement and maintenance costs can vary depending on the specific model and usage conditions, so it is important to evaluate these factors in the context of the intended application .
from Hitachi High-Tech America
Outstanding Performance. Two performance capabilities supporting data reliability: excellent reproducibility made possible by the pump and autosampler and excellent stability of the column oven and detector. Easy-to-use. Along with simple operation and ease of use, Chromaster offers ease of... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Fluorescence; Mass Spectroscopy
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Shimadzu Scientific Instruments, Inc.
Shimadzu's Discovery VP Analytical System enables characterization of microgram to milligram quantities of lead candidates generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, or Organic Synthesis efforts. Up to ten columns and four solvent pairs can be configured, making... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Refractive Index; Light Scattering; Mass Spectroscopy; PDA
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
An economical option for gradient analysis is a quaternary HPLC system with a single pump and valve to mix up to four solvents. The Nexera quaternary system operates at analytical to semi-prep flows (max. 10 mL/min) and throughout the HPLC and UHPLC pressure range, up to 19,000 psi, with no... [See More]
- Instrument Type: Fixtured
- Detector Type: Ultraviolet/Visible Light Detector; Fluorescence
- Analysis Method: Analytical
- Injection Method: Auto-sampler Injection
from Shimadzu Scientific Instruments, Inc.
Realization of Advanced Laboratory. From setting vial to starting analysis, all the procedures are performed via the equipment. The operations in the laboratory are minimized and free of errors. The system status can be supervised even from outside the laboratory. More efficient use of laboratory... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Shimadzu Scientific Instruments, Inc.
Nexera UC improves your analytical workflow by utilizing a completely new separation technology, Unified Chromatography, which unites sample separation, analysis with various separation modes, and high-sensitivity detection. [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump
from Shimadzu Scientific Instruments, Inc.
Method scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method... [See More]
- Instrument Type: Fixtured
- Injection Method: Auto-sampler Injection
- Analysis Method: Analytical
- Flow Control: Gradient Pump